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Literature summary for 2.5.1.7 extracted from

  • Jackson, S.G.; Zhang, F.; Chindemi, P.; Junop, M.S.; Berti, P.J.
    Evidence of kinetic control of ligand binding and staged product release in MurA (enolpyruvyl UDP-GlcNAc synthase)-catalyzed reactions (2009), Biochemistry, 48, 11715-11723.
    View publication on PubMed
Search for more literature for 2.5.1.7

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of wild type MurA Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
MurA is crystallized in the presence of sodium phosphite and UDP-N-acetylmuramic acid using the hanging drop vapor diffusion method, the MurA cocrystal structure with UDP-N-acetylmuramic acid and phosphite reveals a new staged MurA conformation in which the Arg397 side chain tracked phosphite out of the catalytic site Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
fosfomycin enolpyruvyl UDP-GlcNAc synthase is an antimicrobial target that is inhibited by the antibiotic fosfomycin Escherichia coli
UDP-N-acetylmuramic acid
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine Escherichia coli
-
 phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A749
-
-

Purification (Commentary)

Purification (Comment) Organism
on to a HiTrap QP-Sepharose HP ion exchange column Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
 Escherichia coli phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
monomer it appears to be a monomer in solution, even at high concentrations Escherichia coli

Synonyms

Synonyms Comment Organism
enolpyruvyl UDP-GlcNAc synthase
-
 Escherichia coli
MurA
-
 Escherichia coli

General Information

General Information Comment Organism
physiological function catalyzes the first committed step in peptidoglycan biosynthesis Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
520000
-
 additional information MurA-catalyzed breakdown of its tetrahedral intermediate at pH 7.5, second-order rate constant Escherichia coli
570000
-
 additional information MurA-catalyzed breakdown of its tetrahedral intermediate at pH 10.0 Escherichia coli
6000000
-
 additional information MurA-catalyzed breakdown of its tetrahedral intermediate at pH 5.0 Escherichia coli