Cloned (Comment) | Organism |
---|---|
overexpression of wild type MurA | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
MurA is crystallized in the presence of sodium phosphite and UDP-N-acetylmuramic acid using the hanging drop vapor diffusion method, the MurA cocrystal structure with UDP-N-acetylmuramic acid and phosphite reveals a new staged MurA conformation in which the Arg397 side chain tracked phosphite out of the catalytic site | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fosfomycin | enolpyruvyl UDP-GlcNAc synthase is an antimicrobial target that is inhibited by the antibiotic fosfomycin | Escherichia coli | |
UDP-N-acetylmuramic acid | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | Escherichia coli | - |
phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A749 | - |
- |
Purification (Comment) | Organism |
---|---|
on to a HiTrap QP-Sepharose HP ion exchange column | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Escherichia coli | phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | it appears to be a monomer in solution, even at high concentrations | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
enolpyruvyl UDP-GlcNAc synthase | - |
Escherichia coli |
MurA | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | catalyzes the first committed step in peptidoglycan biosynthesis | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
520000 | - |
additional information | MurA-catalyzed breakdown of its tetrahedral intermediate at pH 7.5, second-order rate constant | Escherichia coli | |
570000 | - |
additional information | MurA-catalyzed breakdown of its tetrahedral intermediate at pH 10.0 | Escherichia coli | |
6000000 | - |
additional information | MurA-catalyzed breakdown of its tetrahedral intermediate at pH 5.0 | Escherichia coli |