Cloned (Comment) | Organism |
---|---|
genes murA1 and murA2, DNA and amino acid sequence determination, overexpression of both isoforms in Escherichia coli BL21(DE3) | Streptococcus pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | individually inactivation of the 2 isoforms by allelic replacement shows, that the 2 forms can substitute for each other, a double deletion mutant is not viable | Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
phosphoenolpyruvate | isoform MurA2 | Streptococcus pneumoniae | |
0.037 | - |
phosphoenolpyruvate | isoform MurA1 | Streptococcus pneumoniae | |
0.12 | - |
UDP-GlcNAc | isoform MurA2 | Streptococcus pneumoniae | |
0.244 | - |
UDP-GlcNAc | isoform MurA1 | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | Streptococcus pneumoniae | enzyme activity is essential for the organism, the 2 isoforms can substitute for each other | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Streptococcus pneumoniae | - |
2 genes murA1 and murA2, 2 isoforms of enzyme MurA | - |
Purification (Comment) | Organism |
---|---|
recombinant, 2 isoforms from Escherichia coli | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Streptococcus pneumoniae | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? | |
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | enzyme activity is essential for the organism, the 2 isoforms can substitute for each other | Streptococcus pneumoniae | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | phylogenetic tree | Streptococcus pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
UDP-GlcNAc | isoform MurA1 | Streptococcus pneumoniae | |
0.78 | - |
UDP-GlcNAc | isoform MurA2 | Streptococcus pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptococcus pneumoniae |