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Literature summary for 2.5.1.7 extracted from

  • Wickus, G.G.; Strominger, J.L.
    Partial purification and properties of the pyruvate-uridine diphospho-N-acetylglucosamine transferase from Staphylococcus epidermidis (1973), J. Bacteriol., 113, 287-290.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
EDTA, DTT and dithioerythritol stabilize Staphylococcus epidermidis

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) complete inhibition at 0.01 M; i.e. DTNB Staphylococcus epidermidis
N-ethylmaleimide complete inhibition at 0.2 M Staphylococcus epidermidis
UDP-N-acetylmuramic acid
-
Staphylococcus epidermidis
UDP-N-acetylmuramic acid-L-Ala-D-Glu no inhibition Staphylococcus epidermidis

Organism

Organism UniProt Comment Textmining
Staphylococcus epidermidis
-
-
-
Staphylococcus epidermidis Texas 26
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Staphylococcus epidermidis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
partially purified enzyme Staphylococcus epidermidis

Storage Stability

Storage Stability Organism
-15°C, crude enzyme preparation, in presence of 4 mM DTT, loss of 84% activity within 2 weeks Staphylococcus epidermidis
-196°C, 4 mM DTT, stable for up to 6 weeks, partially purified enzyme Staphylococcus epidermidis
-196°C, slightly increased activity after storage of 2 weeks Staphylococcus epidermidis
-70°C, 4 mM DTT, 22% loss of activity in 2 weeks, partially purified enzyme Staphylococcus epidermidis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
Staphylococcus epidermidis phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine i.e. UDP-N-acetyl-D-glucosamine-enolpyruvate ?
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
Staphylococcus epidermidis Texas 26 phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine i.e. UDP-N-acetyl-D-glucosamine-enolpyruvate ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Staphylococcus epidermidis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
-
Staphylococcus epidermidis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.4
-
Staphylococcus epidermidis