Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thiol groups | required for activity | Enterobacter cloacae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | weak | Enterobacter cloacae | |
Hg2+ | weak | Enterobacter cloacae | |
iodoacetate | - |
Enterobacter cloacae | |
N-ethylmaleimide | - |
Enterobacter cloacae | |
p-chloromercuribenzoate | - |
Enterobacter cloacae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
phosphoenolpyruvate | - |
Enterobacter cloacae | |
0.46 | - |
UDP-GlcNAc | - |
Enterobacter cloacae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by K+, Na+, Mg2+, and Mn2+ | Enterobacter cloacae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | Enterobacter cloacae | pathway for biosynthesis of UDP-N-acetylmuramic acid | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? | |
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | Enterobacter cloacae NRC 492 | pathway for biosynthesis of UDP-N-acetylmuramic acid | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | - |
- |
- |
Enterobacter cloacae NRC 492 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Enterobacter cloacae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.27 | - |
purified enzyme | Enterobacter cloacae |
Storage Stability | Organism |
---|---|
0°C or -20°C, 0.2 mg/ml DTT, 40% loss of activity after 2 weeks | Enterobacter cloacae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | specific for phosphoenolpyruvate and UDP-N-acetyl-D-glucosamine | Enterobacter cloacae | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | i.e. UDP-N-acetyl-D-glucosamine-enolpyruvate | r | |
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | pathway for biosynthesis of UDP-N-acetylmuramic acid | Enterobacter cloacae | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? | |
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | specific for phosphoenolpyruvate and UDP-N-acetyl-D-glucosamine | Enterobacter cloacae NRC 492 | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | i.e. UDP-N-acetyl-D-glucosamine-enolpyruvate | r | |
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | pathway for biosynthesis of UDP-N-acetylmuramic acid | Enterobacter cloacae NRC 492 | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Enterobacter cloacae |
42 | - |
- |
Enterobacter cloacae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | 7.4 | - |
Enterobacter cloacae |