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Literature summary for 2.5.1.69 extracted from
- Enzymes encoded by the farnesyl diphosphate synthase gene family in the big sagebrush Artemisia tridentata ssp. spiciformis (2003), J. Biol. Chem., 278, 32132-32140.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene FDS-5, cDNA library screening, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression of N-terminally His6-tagged enzyme in Escherichia coli strain XA90, expression of the fusion FDS-5 transit peptide-GFP protein in Nicotiana tabacum cv. xanthi cells with plastidial localization | Artemisia spiciformis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plastid | - |
Artemisia spiciformis | 9536 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, 2 mM | Artemisia spiciformis |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 dimethylallyl diphosphate | Artemisia spiciformis | a branching reaction | diphosphate + lavandulyl diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Artemisia spiciformis | Q7XYS8 | big sagebrush | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the pre-enzyme contains an N-terminal plastidial targeting sequence | Artemisia spiciformis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged enzyme from Escherichia coli strain XA90 by nickel affinity chromatography to over 95% purity | Artemisia spiciformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 dimethylallyl diphosphate | reaction of EC 2.5.1.67, chrysanthemyl diphosphate synthase, a cyclopropanation | Artemisia spiciformis | diphosphate + chrysanthemyl diphosphate | an irregular monoterpene product | ? | |
| 2 dimethylallyl diphosphate | a branching reaction | Artemisia spiciformis | diphosphate + lavandulyl diphosphate | - |
? | |
| isopentenyl diphosphate + dimethylallyl diphosphate | reaction of EC 2.5.1.10, geranyltranstransferase, a chain elongation | Artemisia spiciformis | diphosphate + geranyl diphosphate | - |
? | |
| additional information | multifunctional enzyme that catalyzes three competing isoprenoid condensation reactions: chain elongation, cyclopropanation, and branching, substrate specificity, product analysis, overview | Artemisia spiciformis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDS | - |
Artemisia spiciformis |
| FDS-5 | - |
Artemisia spiciformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Artemisia spiciformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Artemisia spiciformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | pH profile | Artemisia spiciformis |
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Release 2023.1 (January 2023)
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