Cloned (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
crystallization using sparse matrix screens and vapor diffusion, structure of apo Rv1086 with citronellyl diphosphate bound and with the product mimic (2E,6E)-farnesyl diphosphate bound. The maximal reaction rate of the wild-type enzyme is 10fold greater than that of the mutant enzyme | Mycobacterium tuberculosis |
in complex with citronellyl diphosphate and with product mimic (E,E)-farnesyl diphosphate | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
L84A | potentially important in limiting chain length. The mutant L84A is not expressed in a soluble form | Mycobacterium tuberculosis |
L84A/L85F | mutant enzyme has a Km-value for geranyl diphosphate of 0.15 mM compared to 0.038 mM for wild-type Rv108612 | Mycobacterium tuberculosis |
L84A/L85F | well expressed and active. Mutant protein is capable of synthesizing prenyl diphosphates containing 20 carbon atoms when geranyl diphosphate is used as the allylic substrate and 50 carbon atoms when EE-farnesyl diphosphate is used as the allylic substrate | Mycobacterium tuberculosis |
V107G | inactive | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
geranyl diphosphate | mutant L84A/L85F | Mycobacterium tuberculosis | |
0.15 | - |
geranyl diphosphate | pH 7.9, 37°C, mutant enzyme L84A/L85F | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WFF5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WFF5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | ? + diphosphate | no substrate for wild-type enzyme. Mutant L84A/L85A synthesizes prenyl diphosphates containing 50 carbon atoms | ? | |
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis H37Rv | ? + diphosphate | no substrate for wild-type enzyme. Mutant L84A/L85A synthesizes prenyl diphosphates containing 50 carbon atoms | ? | |
geranyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | (2Z,6E)-farnesyl diphosphate + diphosphate | product of wild-type.Mutant L84A/L85A synthesizes prenyl diphosphates containing 20 carbon atoms | ? | |
geranyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis H37Rv | (2Z,6E)-farnesyl diphosphate + diphosphate | product of wild-type.Mutant L84A/L85A synthesizes prenyl diphosphates containing 20 carbon atoms | ? | |
geranyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | diphosphate + (2Z,6E)-farnesyl diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis H37Rv | diphosphate + (2Z,6E)-farnesyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration and crystallization data | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
Rv1086 | - |
Mycobacterium tuberculosis |