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Literature summary for 2.5.1.68 extracted from

  • Schulbach, M.C.; Mahapatra, S.; Macchia, M.; Barontini, S.; Papi, C.; Minutolo, F.; Bertini, S.; Brennan, P.J.; Crick, D.C.
    Purification, enzymatic characterization, and inhibition of the Z-farnesyl diphosphate synthase from Mycobacterium tuberculosis (2001), J. Biol. Chem., 276, 11624-11630.
    View publication on PubMed
Search for more literature for 2.5.1.68

Inhibitors

Inhibitors Comment Organism Structure
(1-[[(3E)-4,8-dimethylnona-3,7-dien-1-yl](hydroxy)phosphoryl]-1-ethylpropyl)phosphonic acid
-
 Mycobacterium tuberculosis
(E)-1-ethyl-1-[(4,8-dimethyl-3,7-nonadienyl)hydroxyphosphoryl] propyl phosphonic acid tripotassium salt
-
 Mycobacterium tuberculosis
(E)-geranyl diphosphate analogues design and synthesis of substrate analogue inhibitors, overview Mycobacterium tuberculosis
citronellyl diphosphate
-
 Mycobacterium tuberculosis
EDTA complete inhibition at 10 mM Mycobacterium tuberculosis
Mn2+ optimal at 0.1 mM, the enzyme absolutely requires a divalent cation, inhibition by Mn2+ at higher concentration of 5.0 mM Mycobacterium tuberculosis
additional information no inhibition by dipotassium salt of (E)-1-[(3,7-methyl-2,6-octadienyl)aminocarbonyl] propyl phosphonic acid, dipotassium salt of (E)-1-[[(3,7-methyl-2,6-octadienyl)oxy]aminocarbonyl] propyl phosphonic acid, and dipotassium salt of (E)-2-oxo-2-[(3,7-methyl-2,6-octadienyl)oxy-[amino]ethyl]phosphonic acid Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 (E)-neryl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.038
-
 (E)-geranyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.124
-
 isopentenyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal at 1.0 mM, the enzyme absolutely requires a divalent cation Mycobacterium tuberculosis
Mn2+ optimal at 0.1 mM, the enzyme absolutely requires a divalent cation, inhibition by Mn2+ at higher concentration of 1.0-5.0 mM Mycobacterium tuberculosis
additional information no activation by Zn2+ and Ca2+ up to 5 mM Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E)-geranyl diphosphate + isopentenyl diphosphate Mycobacterium tuberculosis stereospecific reaction (2Z,6E)-farnesyl diphosphate + diphosphate
-
 ?
(E)-geranyl diphosphate + isopentenyl diphosphate Mycobacterium tuberculosis H37Rv stereospecific reaction (2Z,6E)-farnesyl diphosphate + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme in a single step by chitin affinity chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Mycobacterium tuberculosis
0.0007
-
 in presence of 1.0 mM Mg2+ Mycobacterium tuberculosis
0.0014
-
 in presence of 0.1 mM Mn2+ Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-geranyl diphosphate + isopentenyl diphosphate stereospecific reaction Mycobacterium tuberculosis (2Z,6E)-farnesyl diphosphate + diphosphate
-
 ?
(E)-geranyl diphosphate + isopentenyl diphosphate stereospecific reaction Mycobacterium tuberculosis H37Rv (2Z,6E)-farnesyl diphosphate + diphosphate
-
 ?
(E)-neryl diphosphate + isopentenyl diphosphate stereospecific reaction Mycobacterium tuberculosis (2Z,6Z)-farnesyl diphosphate + diphosphate
-
 ?
(E)-neryl diphosphate + isopentenyl diphosphate stereospecific reaction Mycobacterium tuberculosis H37Rv (2Z,6Z)-farnesyl diphosphate + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
omega,E,Z-FPP
-
 Mycobacterium tuberculosis
Z-FPP synthase
-
 Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5
-
 Mycobacterium tuberculosis