Cloned (Comment) | Organism |
---|---|
Rv1086, DNA and amino acid sequence determination and anaylsis, overexpression in Mycobacterium smegmatis cytosol and membranes, the transformed cells show a 5fold increased enzyme activity | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-geranyl diphosphate + isopentenyl diphosphate | Mycobacterium tuberculosis | stereospecific reaction | (2Z,6E)-farnesyl diphosphate + diphosphate | - |
? | |
(E)-geranyl diphosphate + isopentenyl diphosphate | Mycobacterium tuberculosis H37Rv | stereospecific reaction | (2Z,6E)-farnesyl diphosphate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00093 | - |
recombinant Rv1086 in cytosol of Mycobacterium smegmatis | Mycobacterium tuberculosis |
0.00097 | - |
recombinant Rv1086 in membranes of Mycobacterium smegmatis | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-geranyl diphosphate + isopentenyl diphosphate | stereospecific reaction | Mycobacterium tuberculosis | (2Z,6E)-farnesyl diphosphate + diphosphate | - |
? | |
(E)-geranyl diphosphate + isopentenyl diphosphate | stereospecific reaction | Mycobacterium tuberculosis H37Rv | (2Z,6E)-farnesyl diphosphate + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme contains a DD(XX)2D motif, secondary structure analysis | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
Rv1086 | - |
Mycobacterium tuberculosis |
short (C15) chain Z-isoprenyl diphosphate synthase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Mycobacterium tuberculosis |