Reference to 2.5.1.67; Id = 669199 - BRENDA Enzyme Database

gene FDS-5, cDNA library screening, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression of N-terminally His6-tagged enzyme in Escherichia coli strain XA90, expression of the fusion FDS-5 transit peptide-GFP protein in Nicotiana tabacum cv. xanthi cells with plastidial localization

Artemisia spiciformis

plastid

-

Artemisia spiciformis

9536

-

Mg2+

required, 2 mM

Artemisia spiciformis

dimethylallyl diphosphate

Artemisia spiciformis

-

diphosphate + chrysanthemyl diphosphate

-

?

Artemisia spiciformis

Q7XYS8

FDS-5 or ArtCDS; big sagebrush

-

proteolytic modification

the pre-enzyme contains an N-terminal plastidial targeting sequence

Artemisia spiciformis

recombinant N-terminally His6-tagged enzyme from Escherichia coli strain XA90 by nickel affinity chromatography to over 95% purity

Artemisia spiciformis

dimethylallyl diphosphate

-

669199

Artemisia spiciformis

diphosphate + chrysanthemyl diphosphate

-

?

dimethylallyl diphosphate

a cyclopropanation

669199

Artemisia spiciformis

diphosphate + chrysanthemyl diphosphate

an irregular monoterpene product

-

?

dimethylallyl diphosphate

reaction of EC 2.5.1.69, lavandulyl diphosphate synthase, a branching reaction

669199

Artemisia spiciformis

diphosphate + lavandulyl diphosphate

-

?

isopentenyl diphosphate + dimethylallyl diphosphate

reaction of EC 2.5.1.10, geranyltranstransferase, a chain elongation

669199

Artemisia spiciformis

diphosphate + geranyl diphosphate

-

?

additional information

multifunctional enzyme that catalyzes three competing isoprenoid condensation reactions chain elongation, cyclopropanation, and branching, substrate specificity, product analysis, overview

669199

Artemisia spiciformis

?

-

?

CDS

-

Artemisia spiciformis

FDS-5

-

Artemisia spiciformis

37

-

assay at

Artemisia spiciformis

7

-

assay at

Artemisia spiciformis

6

9

pH profile

Artemisia spiciformis

gene FDS-5, cDNA library screening, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression of N-terminally His6-tagged enzyme in Escherichia coli strain XA90, expression of the fusion FDS-5 transit peptide-GFP protein in Nicotiana tabacum cv. xanthi cells with plastidial localization

Artemisia spiciformis

plastid

-

Artemisia spiciformis

9536

-

Mg2+

required, 2 mM

Artemisia spiciformis

dimethylallyl diphosphate

Artemisia spiciformis

-

diphosphate + chrysanthemyl diphosphate

-

?

proteolytic modification

the pre-enzyme contains an N-terminal plastidial targeting sequence

Artemisia spiciformis

recombinant N-terminally His6-tagged enzyme from Escherichia coli strain XA90 by nickel affinity chromatography to over 95% purity

Artemisia spiciformis

dimethylallyl diphosphate

-

669199

Artemisia spiciformis

diphosphate + chrysanthemyl diphosphate

-

?

dimethylallyl diphosphate

a cyclopropanation

669199

Artemisia spiciformis

diphosphate + chrysanthemyl diphosphate

an irregular monoterpene product

-

?

dimethylallyl diphosphate

reaction of EC 2.5.1.69, lavandulyl diphosphate synthase, a branching reaction

669199

Artemisia spiciformis

diphosphate + lavandulyl diphosphate

-

?

isopentenyl diphosphate + dimethylallyl diphosphate

reaction of EC 2.5.1.10, geranyltranstransferase, a chain elongation

669199

Artemisia spiciformis

diphosphate + geranyl diphosphate

-

?

additional information

multifunctional enzyme that catalyzes three competing isoprenoid condensation reactions chain elongation, cyclopropanation, and branching, substrate specificity, product analysis, overview

669199

Artemisia spiciformis

?

-

?

37

-

assay at

Artemisia spiciformis

7

-

assay at

Artemisia spiciformis

6

9

pH profile

Artemisia spiciformis

759718

Xu

Production of trans-chrysanth ...

Tanacetum cinerariifolium

Metab. Eng.

47

271-278

2018

-

1

-

7

-

1

-

759925

Hu

Modification of chrysanthemum ...

Tanacetum cinerariifolium

Plant Biotechnol. J.

16

1434-1445

2018

-

12

-

1

-

759979

Xu

Coexpression analysis identif ...

Tanacetum cinerariifolium

Plant Physiol.

176

524-537

2018

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

759401

Lee

Structure-function studies of ...

Artemisia spiciformis

J. Am. Chem. Soc.

139

14556-14567

2017

-

1

-

2

-

3

-

5

-

5

-

2

-

1

-

1

-

2

-

3

-

5

-

1

-

1

-

1

739371

Sakamori

Selective regulation of pyreth ...

Tanacetum cinerariifolium

Plant Signal. Behav.

11

e1149675

2016

-

1

-

1

-

1

-

2

-

2

-

1

-

1

-

2

-

2

-

1

-

738635

Yang

Chrysanthemyl diphosphate synt ...

Tanacetum cinerariifolium

J. Biol. Chem.

289

36325-36335

2014

-

1

-

2

-

1

-

2

-

2

-

1

-

4

-

2

1

-

1

-

1

-

2

-

1

-

1

-

2

-

1

-

4

-

1

-

1

-

4

-

723414

Tang

-

Cloning and functional analysi ...

Tanacetum cinerariifolium

Plant Growth Regul.

67

161-169

2012

-

1

-

4

-

1

-

4

-

1

-

687301

Thulasiram

A common mechanism for branchi ...

Artemisia spiciformis

J. Am. Chem. Soc.

130

1966-1971

2008

-

1

-

1

-

1

-

2

-

2

-

3

1

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

1

-

670890

Christianson

Roots of biosynthetic diversit ...

Artemisia spiciformis

Science

316

60-61

2007

-

1

-

1

-

1

-

1

-

2

1

-

1

-

1

-

1

-

2

1

-

669041

Erickson

Chrysanthemyl diphosphate synt ...

Tanacetum cinerariifolium

J. Am. Chem. Soc.

125

6886-6888

2003

-

3

-

1

-

2

-

1

-

1

-

2

-

1

-

3

-

1

-

2

-

2

-

669199

Hemmerlin

Enzymes encoded by the farnesy ...

Artemisia spiciformis

J. Biol. Chem.

278

32132-32140

2003

-

1

-

1

-

1

-

3

-

1

-

5

-

2

1

-

1

-

1

-

1

-

1

-

1

-

5

-

1

-

1

-

670719

Rivera

Chrysanthemyl diphosphate synt ...

Tanacetum cinerariifolium

Proc. Natl. Acad. Sci. USA

98

4373-4378

2001

-

1

-

1

2

4

2

-

10

-

1

-

1

4

1

-

1

-

1

-

1

2

4

2

-

1

-

1

4

1

-

1

-

Enzymes encoded by the farnesyl diphosphate synthase gene family in the big sagebrush Artemisia tridentata ssp. spiciformis

Data extracted from this reference: