BRENDA - Enzyme Database
show all sequences of 2.5.1.67

Enzymes encoded by the farnesyl diphosphate synthase gene family in the big sagebrush Artemisia tridentata ssp. spiciformis

Hemmerlin, A.; Rivera, S.B.; Erickson, H.K.; Poulter, C.D.; J. Biol. Chem. 278, 32132-32140 (2003) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene FDS-5, cDNA library screening, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression of N-terminally His6-tagged enzyme in Escherichia coli strain XA90, expression of the fusion FDS-5 transit peptide-GFP protein in Nicotiana tabacum cv. xanthi cells with plastidial localization
Artemisia spiciformis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
plastid
-
Artemisia spiciformis
9536
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required, 2 mM
Artemisia spiciformis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
dimethylallyl diphosphate
Artemisia spiciformis
-
diphosphate + chrysanthemyl diphosphate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Artemisia spiciformis
Q7XYS8
FDS-5 or ArtCDS; big sagebrush
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
proteolytic modification
the pre-enzyme contains an N-terminal plastidial targeting sequence
Artemisia spiciformis
Purification (Commentary)
Purification (Commentary)
Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain XA90 by nickel affinity chromatography to over 95% purity
Artemisia spiciformis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
dimethylallyl diphosphate
-
669199
Artemisia spiciformis
diphosphate + chrysanthemyl diphosphate
-
-
-
?
dimethylallyl diphosphate
a cyclopropanation
669199
Artemisia spiciformis
diphosphate + chrysanthemyl diphosphate
an irregular monoterpene product
-
-
?
dimethylallyl diphosphate
reaction of EC 2.5.1.69, lavandulyl diphosphate synthase, a branching reaction
669199
Artemisia spiciformis
diphosphate + lavandulyl diphosphate
-
-
-
?
isopentenyl diphosphate + dimethylallyl diphosphate
reaction of EC 2.5.1.10, geranyltranstransferase, a chain elongation
669199
Artemisia spiciformis
diphosphate + geranyl diphosphate
-
-
-
?
additional information
multifunctional enzyme that catalyzes three competing isoprenoid condensation reactions chain elongation, cyclopropanation, and branching, substrate specificity, product analysis, overview
669199
Artemisia spiciformis
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CDS
-
Artemisia spiciformis
FDS-5
-
Artemisia spiciformis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Artemisia spiciformis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Artemisia spiciformis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9
pH profile
Artemisia spiciformis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene FDS-5, cDNA library screening, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression of N-terminally His6-tagged enzyme in Escherichia coli strain XA90, expression of the fusion FDS-5 transit peptide-GFP protein in Nicotiana tabacum cv. xanthi cells with plastidial localization
Artemisia spiciformis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
plastid
-
Artemisia spiciformis
9536
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required, 2 mM
Artemisia spiciformis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
dimethylallyl diphosphate
Artemisia spiciformis
-
diphosphate + chrysanthemyl diphosphate
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
proteolytic modification
the pre-enzyme contains an N-terminal plastidial targeting sequence
Artemisia spiciformis
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain XA90 by nickel affinity chromatography to over 95% purity
Artemisia spiciformis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
dimethylallyl diphosphate
-
669199
Artemisia spiciformis
diphosphate + chrysanthemyl diphosphate
-
-
-
?
dimethylallyl diphosphate
a cyclopropanation
669199
Artemisia spiciformis
diphosphate + chrysanthemyl diphosphate
an irregular monoterpene product
-
-
?
dimethylallyl diphosphate
reaction of EC 2.5.1.69, lavandulyl diphosphate synthase, a branching reaction
669199
Artemisia spiciformis
diphosphate + lavandulyl diphosphate
-
-
-
?
isopentenyl diphosphate + dimethylallyl diphosphate
reaction of EC 2.5.1.10, geranyltranstransferase, a chain elongation
669199
Artemisia spiciformis
diphosphate + geranyl diphosphate
-
-
-
?
additional information
multifunctional enzyme that catalyzes three competing isoprenoid condensation reactions chain elongation, cyclopropanation, and branching, substrate specificity, product analysis, overview
669199
Artemisia spiciformis
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Artemisia spiciformis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Artemisia spiciformis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9
pH profile
Artemisia spiciformis
Other publictions for EC 2.5.1.67
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
759718
Xu
Production of trans-chrysanth ...
Tanacetum cinerariifolium
Metab. Eng.
47
271-278
2018
-
1
-
-
-
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7
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1
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-
-
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759925
Hu
Modification of chrysanthemum ...
Tanacetum cinerariifolium
Plant Biotechnol. J.
16
1434-1445
2018
-
-
-
-
-
-
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12
-
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1
1
-
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-
759979
Xu
Coexpression analysis identif ...
Tanacetum cinerariifolium
Plant Physiol.
176
524-537
2018
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
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1
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1
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1
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1
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1
-
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-
-
-
-
-
-
-
-
-
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-
759401
Lee
Structure-function studies of ...
Artemisia spiciformis
J. Am. Chem. Soc.
139
14556-14567
2017
-
-
-
-
1
-
-
2
-
-
-
3
-
5
-
-
-
-
-
-
-
-
5
-
2
-
-
-
1
-
-
-
-
-
-
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-
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-
1
-
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-
2
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-
3
-
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-
-
-
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-
-
5
-
-
-
-
1
-
-
-
-
-
1
1
-
1
1
739371
Sakamori
Selective regulation of pyreth ...
Tanacetum cinerariifolium
Plant Signal. Behav.
11
e1149675
2016
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
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1
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1
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2
-
-
2
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
738635
Yang
Chrysanthemyl diphosphate synt ...
Tanacetum cinerariifolium
J. Biol. Chem.
289
36325-36335
2014
-
-
1
-
2
-
1
1
1
1
-
2
-
2
-
-
1
-
-
-
-
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4
-
2
1
-
-
-
1
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-
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-
1
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2
-
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1
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1
1
1
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2
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1
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4
-
1
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-
1
-
-
-
-
4
4
-
-
-
723414
Tang
-
Cloning and functional analysi ...
Tanacetum cinerariifolium
Plant Growth Regul.
67
161-169
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
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1
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4
-
-
-
-
-
-
-
-
-
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-
-
1
1
1
1
-
-
687301
Thulasiram
A common mechanism for branchi ...
Artemisia spiciformis
J. Am. Chem. Soc.
130
1966-1971
2008
-
-
1
-
-
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
-
2
-
3
1
-
-
-
1
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
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1
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1
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-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
670890
Christianson
Roots of biosynthetic diversit ...
Artemisia spiciformis
Science
316
60-61
2007
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
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-
-
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669041
Erickson
Chrysanthemyl diphosphate synt ...
Tanacetum cinerariifolium
J. Am. Chem. Soc.
125
6886-6888
2003
-
-
-
-
3
-
-
1
-
-
-
2
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
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3
-
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1
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2
-
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-
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669199
Hemmerlin
Enzymes encoded by the farnesy ...
Artemisia spiciformis
J. Biol. Chem.
278
32132-32140
2003
-
-
1
-
-
-
-
-
1
1
-
1
-
3
-
1
1
-
-
-
-
-
5
-
2
1
-
-
-
1
1
-
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1
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1
1
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1
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1
1
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-
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5
-
1
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1
1
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-
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-
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-
670719
Rivera
Chrysanthemyl diphosphate synt ...
Tanacetum cinerariifolium
Proc. Natl. Acad. Sci. USA
98
4373-4378
2001
-
-
1
-
-
-
-
1
1
2
4
2
-
10
-
1
1
-
-
1
1
1
4
1
1
1
-
-
1
1
-
-
-
-
-
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1
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-
-
-
-
-
1
1
2
4
2
-
-
1
1
-
1
1
1
4
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-