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Literature summary for 2.5.1.65 extracted from

  • OLeary, S.E.; Jurgenson, C.T.; Ealick, S.E.; Begley, T.P.
    O-Phospho-l-serine and the thiocarboxylated sulfur carrier protein CysO-COSH are substrates for CysM, a cysteine synthase from Mycobacterium tuberculosis (2008), Biochemistry, 47, 11606-11615.
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
isoform CysM
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information specificity of CysM for its amino acid substrate is more than 500-fold greater for O-phospho-L-serine than for O-acetyl-L-serine. Specificity of CysM for this physiological sulfide equivalent, sulfur carrier protein CysO-COSH, is more than 3 orders of magnitude greater than that for bisulfide. CysM active site with the bound aminoacrylate intermediate is protected from solvent and that binding of CysO-COSH produces a conformational change allowing rapid sulfur transfer Mycobacterium tuberculosis ?
-
?
O-acetyl-L-serine + hydrogen sulfide
-
Mycobacterium tuberculosis L-cysteine + acetate
-
?
O3-phospho-L-serine + hydrogen sulfide
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Mycobacterium tuberculosis L-cysteine + phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.025
-
O3-acetyl-L-serine formation of the aminoacrylate intermediate Mycobacterium tuberculosis
17
-
O3-phospho-L-serine formation of the aminoacrylate intermediate Mycobacterium tuberculosis