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Literature summary for 2.5.1.63 extracted from

  • Sergeev, M.; Morgia, F.; Javed, M.; Doi, M.; Keng, P.
    Polymer-immobilized fluorinase: recyclable catalyst for fluorination reactions (2013), J. Mol. Catal. B, 92, 51-56.
No PubMed abstract available
Search for more literature for 2.5.1.63

Cloned(Commentary)

Cloned (Comment) Organism
gene flA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-Gold (DE3) pLysS Streptomyces cattleya

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the purified recombinant enzyme on poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) (2:1) polymer monoliths, method development to imporve immobilization yields and enzyme activity, determination of stability of the immobilized enzyme and enzyme leakage, overview. The enzyme shows over 80% activity during 4 reaction cycles, and 20% activity remaining after the 6th cycle Streptomyces cattleya

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + fluoride Streptomyces cattleya
-
 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?
S-adenosyl-L-methionine + fluoride Streptomyces cattleya NBRC14057
-
 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces cattleya F8JPG4 gene flA
-
Streptomyces cattleya NBRC14057 F8JPG4 gene flA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold (DE3) pLysS by nickel affinity chromatography and gel filtration Streptomyces cattleya

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + fluoride
-
 Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?
S-adenosyl-L-methionine + fluoride
-
 Streptomyces cattleya NBRC14057 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?

Synonyms

Synonyms Comment Organism
fluorinase
-
 Streptomyces cattleya

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
 assay at Streptomyces cattleya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Streptomyces cattleya