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Literature summary for 2.5.1.63 extracted from
- Characterization of a SAM-dependent fluorinase from a latent biosynthetic pathway for fluoroacetate and 4-fluorothreonine formation in Nocardia brasiliensis (2014), F1000Res., 3, 61-73.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Nocardia brasiliensis |  |
| EDTA | - |
Streptomyces cattleya | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene flA, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type His6-tagged enzymes in Escherichia coli strain BL21(DE3) | Streptomyces cattleya |
| gene nobA, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type and mutant His6-tagged enzymes in Escherichia coli strain BL21(DE3) | Nocardia brasiliensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S158A | site-directed mutagenesis, recombinant mutant protein NobA-S158A completely loses fluorination activity | Nocardia brasiliensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Nocardia brasiliensis | |
| Fe2+ | - |
Nocardia brasiliensis | |
| Zn2+ | - |
Nocardia brasiliensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
S-adenosyl-L-methionine | pH 7.5, 26°C, recombinant enzyme | Streptomyces cattleya | |
| 0.416 | - |
S-adenosyl-L-methionine | pH 7.5, 26°C, recombinant enzyme | Nocardia brasiliensis | |
| 2.167 | - |
fluoride | pH 7.5, 26°C, recombinant enzyme | Streptomyces cattleya | |
| 4.153 | - |
fluoride | pH 7.5, 26°C, recombinant enzyme | Nocardia brasiliensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | slightly activating | Nocardia brasiliensis | |
| Mn2+ | slightly activating | Nocardia brasiliensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34600 | - |
- |
Streptomyces cattleya |
| 34700 | - |
- |
Nocardia brasiliensis |
| 200000 | - |
recombinant enzyme, gel filtration | Nocardia brasiliensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + fluoride | Nocardia brasiliensis | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
| S-adenosyl-L-methionine + fluoride | Streptomyces cattleya | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
| S-adenosyl-L-methionine + fluoride | Nocardia brasiliensis ATCC 700358 | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
| S-adenosyl-L-methionine + fluoride | Streptomyces cattleya DSM 46488 | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nocardia brasiliensis | W8JNL4 | gene nobA | - |
| Nocardia brasiliensis ATCC 700358 | W8JNL4 | gene nobA | - |
| Streptomyces cattleya | Q70GK9 | gene flA | - |
| Streptomyces cattleya DSM 46488 | Q70GK9 | gene flA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Nocardia brasiliensis |
| recombinant wild-type His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Streptomyces cattleya |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + fluoride | - |
Nocardia brasiliensis | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
| S-adenosyl-L-methionine + fluoride | - |
Streptomyces cattleya | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
| S-adenosyl-L-methionine + fluoride | - |
Nocardia brasiliensis ATCC 700358 | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
| S-adenosyl-L-methionine + fluoride | - |
Streptomyces cattleya DSM 46488 | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34600 recombinant enzyme, SDS-PAGE | Streptomyces cattleya |
| hexamer | 6 * 34700 recombinant enzyme, SDS-PAGE | Nocardia brasiliensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| flA | - |
Streptomyces cattleya |
| fluorinase | - |
Nocardia brasiliensis |
| fluorinase | - |
Streptomyces cattleya |
| NobA | - |
Nocardia brasiliensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
the optimal temperature for fluorination reactivity is 37°C. At this temperature S-adenosyl-L-methionine degradation is also expedited. 26°C can provide acceptable activity and slow down the degradation of S-adenosyl-L-methionine and is therefore used for kinetic studies | Nocardia brasiliensis |
| 37 | - |
the optimal temperature for fluorination reactivity is 37°C. At this temperature S-adenosyl-L-methionine degradation is also expedited. 26°C can provide acceptable activity and slow down the degradation of S-adenosyl-L-methionine and is therefore used for kinetic studies | Streptomyces cattleya |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
fluoride | pH 7.5, 26°C, recombinant enzyme | Nocardia brasiliensis | |
| 0.0018 | - |
fluoride | pH 7.5, 26°C, recombinant enzyme | Streptomyces cattleya | |
| 0.0021 | - |
S-adenosyl-L-methionine | pH 7.5, 26°C, recombinant enzyme | Streptomyces cattleya | |
| 0.0023 | - |
S-adenosyl-L-methionine | pH 7.5, 26°C, recombinant enzyme | Nocardia brasiliensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Nocardia brasiliensis |
| 7 | - |
- |
Streptomyces cattleya |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.5 | activity range, profile overview | Nocardia brasiliensis |
| 5.5 | 10 | activity range, profile overview | Streptomyces cattleya |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the fluorinases group of the DUF62 enzyme family | Nocardia brasiliensis |
| evolution | the enzyme belongs to the fluorinases group of the DUF62 enzyme family | Streptomyces cattleya |
| metabolism | enzyme NobA shows similar halide specificity and characteristics to the fluorination enzyme FlA of the fl pathway | Nocardia brasiliensis |
| metabolism | enzyme NobA shows similar halide specificity and characteristics to the fluorination enzyme FlA of the fl pathway | Streptomyces cattleya |
| additional information | essential residue S158 of FlA plays critical role in fluoride binding and desolvation | Streptomyces cattleya |
| additional information | essential residue S158 of NbA is required for activity | Nocardia brasiliensis |
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Release 2023.1 (January 2023)
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