Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is applicated in positron emission tomography (PET) as a result of its ability to catalyze C-18F bond formation in the presence of [18F]fluoride as the nucleophile. The enzymatic process has a technical advantage in the PET context because [18F]fluoride is generated in the cyclotron as a dilute solution in [18O]water, and the enzyme can use this form of aqueous fluoride directly. This feature obviates the usual requirement to secure dry [18F]fluoride by ion-exchange chromatography. The enzymatic process therefore offers the possibility to directly radiolabel biomolecules in the aqueous phase | Streptomyces cattleya |
Crystallization (Comment) | Organism |
---|---|
purified enzyme in complex with 5'-fluorodeoxy-2-ethynyladenosine, X-ray diffraction structure determination and analysis at 2.4 A resolution, molecular replacement | Streptomyces cattleya |
Protein Variants | Comment | Organism |
---|---|---|
additional information | engineering of the enzyme by substrate modulation: An acetylene functionality at the C-2 position of the adenosine substrate projects from the active site into the solvent, for 18F fluorination of bioconjugated peptides by the enzyme, extending a polyethylene glycol linker from the terminus of the acetylene allows the presentation of bioconjugation cargo to the enzyme for 18F labelling, method development and evaluation, overview | Streptomyces cattleya |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + fluoride | Streptomyces cattleya | - |
5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces cattleya | Q70GK9 | gene flA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the fluorinase catalyzes a transhalogenation of 5'-chlorodeoxy-2-ethynyladenosine to 5'-fluorodeoxy-2-ethynyladenosine, substrate specificity, the enzyme does have a specificity weakness at the C-2 position of adenine, overview | Streptomyces cattleya | ? | - |
? | |
S-adenosyl-L-methionine + fluoride | - |
Streptomyces cattleya | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
fluorinase | - |
Streptomyces cattleya |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptomyces cattleya |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces cattleya |