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Literature summary for 2.5.1.63 extracted from

  • Thompson, S.; Zhang, Q.; Onega, M.; McMahon, S.; Fleming, I.; Ashworth, S.; Naismith, J.H.; Passchier, J.; O'Hagan, D.
    A localized tolerance in the substrate specificity of the fluorinase enzyme enables last-step 18F fluorination of a RGD peptide under ambient aqueous conditions (2014), Angew. Chem. Int. Ed. Engl., 53, 8913-8918.
    View publication on PubMed
Search for more literature for 2.5.1.63

Application

Application Comment Organism
synthesis the enzyme is applicated in positron emission tomography (PET) as a result of its ability to catalyze C-18F bond formation in the presence of [18F]fluoride as the nucleophile. The enzymatic process has a technical advantage in the PET context because [18F]fluoride is generated in the cyclotron as a dilute solution in [18O]water, and the enzyme can use this form of aqueous fluoride directly. This feature obviates the usual requirement to secure dry [18F]fluoride by ion-exchange chromatography. The enzymatic process therefore offers the possibility to directly radiolabel biomolecules in the aqueous phase Streptomyces cattleya

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme in complex with 5'-fluorodeoxy-2-ethynyladenosine, X-ray diffraction structure determination and analysis at 2.4 A resolution, molecular replacement Streptomyces cattleya

Protein Variants

Protein Variants Comment Organism
additional information engineering of the enzyme by substrate modulation: An acetylene functionality at the C-2 position of the adenosine substrate projects from the active site into the solvent, for 18F fluorination of bioconjugated peptides by the enzyme, extending a polyethylene glycol linker from the terminus of the acetylene allows the presentation of bioconjugation cargo to the enzyme for 18F labelling, method development and evaluation, overview Streptomyces cattleya

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + fluoride Streptomyces cattleya
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 5'-deoxy-5'-fluoroadenosine + L-methionine
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces cattleya Q70GK9 gene flA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the fluorinase catalyzes a transhalogenation of 5'-chlorodeoxy-2-ethynyladenosine to 5'-fluorodeoxy-2-ethynyladenosine, substrate specificity, the enzyme does have a specificity weakness at the C-2 position of adenine, overview Streptomyces cattleya ?
-
 ?
S-adenosyl-L-methionine + fluoride
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 Streptomyces cattleya 5'-deoxy-5'-fluoroadenosine + L-methionine
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 ?

Synonyms

Synonyms Comment Organism
fluorinase
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 Streptomyces cattleya

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Streptomyces cattleya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Streptomyces cattleya