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Literature summary for 2.5.1.61 extracted from

  • Huang, M.; Slewinski, T.L.; Baker, R.F.; Janick-Buckner, D.; Buckner, B.; Johal, G.S.; Braun, D.M.
    Camouflage patterning in maize leaves results from a defect in porphobilinogen deaminase (2009), Mol. Plant, 2, 773-789.
    View publication on PubMed
Search for more literature for 2.5.1.61

Cloned(Commentary)

Cloned (Comment) Organism
Lambda ZAP Express system Zea mays

Protein Variants

Protein Variants Comment Organism
additional information 4 insertion allels (m1-m4) of camouflage1 (cf1) mutant, mutant develops nonclonal, yellow-green sectors in leaves based on bundle sheath cell-specific death, yellow mutant regions show reduced levels of chlorophyll a + b, and total carotenoids, reduced photosynthesis and stomatal conductance compared to wild type and green regions of the mutant, constant light suppresses the cf1 sector formation, sectors only form during a limited time of leaf development, underlying is a decreased enzyme activity and increased levels of the substrate in green and yellow regions, yellow regions show an additional reduction in catalase activity Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast fusion enzyme with yellow fluorescent protein (YFP), transformed in Vicia faba leaves for localization analysis Zea mays 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4 porphobilinogen + H2O Zea mays
-
 hydroxymethylbilane + 4 NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Zea mays
-
 maize
-

Purification (Commentary)

Purification (Comment) Organism
leaves of 8 week old field grown plants are ground in liquid nitrogen and extracted with 25 mM NaP buffer, pH 8.0, containing 1 mM EDTA, 5 mM DTT, and 0.5 mM phenylmethanesulfonylfluoride, centrifuged, supernatant taken for enzyme assay Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
fruit in developing ears Zea mays
-
leaf mature and etiolated leaves Zea mays
-
root seedling roots Zea mays
-
tassel in developing tassels Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 cf1 mutant tissue shows 30% reduced activity (about 0.7 units/mg) compared to wild type (about 2.5 units/mg) in green and yellow leaf tissue, consistent with reduced enzyme abundance, 13fold substrate accumulation Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 porphobilinogen + H2O
-
 Zea mays hydroxymethylbilane + 4 NH3
-
 ?

Synonyms

Synonyms Comment Organism
PBGD
-
 Zea mays
porphobilinogen deaminase
-
 Zea mays

Expression

Organism Comment Expression
Zea mays cf1 mutation show strongly decreased expression compared to wild type down

General Information

General Information Comment Organism
malfunction camouflage1 (cf1) mutant develops nonclonal, yellow-green sectors in leaves based on bundle sheath cell-specific death, yellow mutant regions show reduced levels of chlorophyll a + b, and total carotenoids, reduced photosynthesis and stomatal conductance compared to wild type and green regions of the mutant, constant light suppresses the cf1 sector formation, sectors only form during a limited time of leaf development, underlying is a decreased enzyme activity and increased levels of the substrate in green and yellow regions, yellow regions show an additional reduction in catalase activity Zea mays
physiological function early step in chlorophyll and heme biosynthesis Zea mays