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Literature summary for 2.5.1.61 extracted from
- Studies on porphobilinogen deaminase and uroporphyrinogen III cosynthase from human erythrocytes (1974), Biochim. Biophys. Acta, 350, 358-373.
General Stability
| General Stability | Organism |
|---|---|
| phosphate stabilizes | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-methylopsopyrroledicarboxylic acid | - |
Homo sapiens |  |
| Ca2+ | 25 mM CaCl2, 90% inhibition | Homo sapiens | |
| Cd2+ | 12 mM, complete inhibition | Homo sapiens | |
| glycerol | 15% inhibits 40% of enzyme activity | Homo sapiens | |
| isoporphobilinogen | - |
Homo sapiens | |
| Mg2+ | 25 mM, 90% inhibition | Homo sapiens | |
| N-ethylmaleimide | - |
Homo sapiens | |
| p-hydroxymercuribenzoate | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.046 | - |
porphobilinogen | porphobilinogen deaminase/uroporphyrinogen III cosynthase enzyme system | Homo sapiens | |
| 0.051 | - |
Hydroxymethylbilane | porphobilinogen deaminase/uroporphyrinogen III cosynthase enzyme system | Homo sapiens | |
| 0.077 | - |
Hydroxymethylbilane | - |
Homo sapiens | |
| 0.13 | - |
porphobilinogen | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| porphobilinogen | Homo sapiens | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| photooxidation of the enzyme in the presence of methylene blue, 0.03%, and roes bengal, 0.03%, over a 5 min period, inhibits 90% and 60% of the enzyme activity, respectively | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, purified enzyme rapidely loses its activity | Homo sapiens |
| 4°C, purified enzyme stable for more than a month | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Homo sapiens | uroporphyrinogen III + 4 NH3 | - |
? | |
| 4 porphobilinogen + H2O | PBG | Homo sapiens | hydroxylmethylbilane + 4 NH3 | - |
? | |
| porphobilinogen | - |
Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| porphobilinogen deaminase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
activity twice that at 37°C | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
| 65 | - |
15 min, no loss of activity | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
phosphate buffer | Homo sapiens |
| 8.2 | - |
Tris-HCl buffer | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | pH 5.0: unstable below, pH 9.0 stable above | Homo sapiens |
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