Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aniline | - |
Cereibacter sphaeroides | |
Ca2+ | - |
Cereibacter sphaeroides | |
hydrazine | - |
Cereibacter sphaeroides | |
hydroxylamine | - |
Cereibacter sphaeroides | |
methoxyamine | - |
Cereibacter sphaeroides | |
methylamine | - |
Cereibacter sphaeroides | |
Mg2+ | - |
Cereibacter sphaeroides | |
additional information | mechanism of inhibition | Cereibacter sphaeroides | |
additional information | - |
Triticum aestivum | |
NH4+ | - |
Cereibacter sphaeroides | |
opsopyrroledicarboxylic acid | 1 mM, 40% inhibition | Cereibacter sphaeroides | |
opsopyrroledicarboxylic acid | - |
Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
porphobilinogen | - |
Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration | Cereibacter sphaeroides |
36000 | - |
1 * 36000, SDS-PAGE in the presence of beta-mercaptoethanol | Cereibacter sphaeroides |
36000 | - |
1 * 36000, SDS-PAGE in the presence and in the absence of 2-mercaptoethanol | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
porphobilinogen | Bos taurus | - |
? | - |
? | |
porphobilinogen | Triticum aestivum | - |
? | - |
? | |
porphobilinogen | Cereibacter sphaeroides | - |
? | - |
? | |
porphobilinogen | Spinacia oleracea | - |
? | - |
? | |
porphobilinogen | Glycine max | - |
? | - |
? | |
porphobilinogen | Cereibacter sphaeroides NCIB 8253 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Cereibacter sphaeroides | - |
N.C.I.B. 8253 | - |
Cereibacter sphaeroides NCIB 8253 | - |
N.C.I.B. 8253 | - |
Glycine max | - |
soya-bean | - |
Spinacia oleracea | - |
spinach | - |
Triticum aestivum | - |
wheat | - |
Purification (Comment) | Organism |
---|---|
- |
Cereibacter sphaeroides |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus | - |
Bos taurus | - |
callus | - |
Glycine max | - |
germ | - |
Triticum aestivum | - |
leaf | - |
Spinacia oleracea | - |
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Cereibacter sphaeroides |
0.45 | - |
- |
Cereibacter sphaeroides |
Storage Stability | Organism |
---|---|
-20°C, stable for over 1 year | Cereibacter sphaeroides |
0°C, stable in solution at pH 7.5 and 9.5 for 3 weeks | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Bos taurus | uroporphyrinogen III + 4 NH3 | - |
? | |
4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Triticum aestivum | uroporphyrinogen III + 4 NH3 | - |
? | |
4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Cereibacter sphaeroides | uroporphyrinogen III + 4 NH3 | - |
? | |
4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Spinacia oleracea | uroporphyrinogen III + 4 NH3 | - |
? | |
4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Glycine max | uroporphyrinogen III + 4 NH3 | - |
? | |
4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Cereibacter sphaeroides NCIB 8253 | uroporphyrinogen III + 4 NH3 | - |
? | |
4 porphobilinogen + H2O | - |
Bos taurus | hydroxylmethylbilane + 4 NH3 | - |
? | |
4 porphobilinogen + H2O | - |
Triticum aestivum | hydroxylmethylbilane + 4 NH3 | - |
? | |
4 porphobilinogen + H2O | - |
Spinacia oleracea | hydroxylmethylbilane + 4 NH3 | - |
? | |
4 porphobilinogen + H2O | - |
Glycine max | hydroxylmethylbilane + 4 NH3 | - |
? | |
4 porphobilinogen + H2O | - |
Cereibacter sphaeroides | hydroxylmethylbilane + 4 NH3 | at pH 8.2 | ? | |
4 porphobilinogen + H2O | - |
Cereibacter sphaeroides | hydroxylmethylbilane + 4 NH3 | uroporphyrinogen I | ? | |
4 porphobilinogen + H2O | - |
Cereibacter sphaeroides NCIB 8253 | hydroxylmethylbilane + 4 NH3 | at pH 8.2 | ? | |
4 porphobilinogen + H2O | - |
Cereibacter sphaeroides NCIB 8253 | hydroxylmethylbilane + 4 NH3 | uroporphyrinogen I | ? | |
porphobilinogen | - |
Bos taurus | ? | - |
? | |
porphobilinogen | - |
Triticum aestivum | ? | - |
? | |
porphobilinogen | - |
Cereibacter sphaeroides | ? | - |
? | |
porphobilinogen | - |
Spinacia oleracea | ? | - |
? | |
porphobilinogen | - |
Glycine max | ? | - |
? | |
porphobilinogen | - |
Cereibacter sphaeroides NCIB 8253 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, SDS-PAGE in the presence of beta-mercaptoethanol | Cereibacter sphaeroides |
monomer | 1 * 36000, SDS-PAGE in the presence and in the absence of 2-mercaptoethanol | Cereibacter sphaeroides |
Synonyms | Comment | Organism |
---|---|---|
porphobilinogen deaminase | - |
Cereibacter sphaeroides |
uroporphyrinogen I synthetase | - |
Cereibacter sphaeroides |
uroporphyrinogen I synthetase | - |
Spinacia oleracea |
uroporphyrinogen synthetase | - |
Cereibacter sphaeroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cereibacter sphaeroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8 | 0.1-0.4 mM porphobilinogen | Cereibacter sphaeroides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.2 | 50% of maximal activity at pH 6.5 and pH 9.2 | Cereibacter sphaeroides |