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Literature summary for 2.5.1.6 extracted from

  • Wang, X.; Jiang, Y.; Wu, M.; Zhu, L.; Yang, L.; Lin, J.
    Semi-rationally engineered variants of S-adenosylmethionine synthetase from Escherichia coli with reduced product inhibition and improved catalytic activity (2019), Enzyme Microb. Technol., 129, 109355 .
    View publication on PubMed
Search for more literature for 2.5.1.6

Protein Variants

Protein Variants Comment Organism
I303V/I65V/L186V product inhibition of the enzyme is reduced via semi-rational modification. The mutant enzyme shows a 42fold increase in Ki(ATP) and a 2.08fold increase in specific activity when compared to wild-type enzyme. Its Ki(ATP) is 0.42 mM and specific acitivity is 3.78 U/mg. Increased Ki(ATP) means reduced product inhibition which enhances accumulation of S-adenosyl-L-methionine. The S-adenosyl-L-methionine produced by the variant could reach to 3.27 mM while S-adenosyl-L-methionine produced by wild-type enzyme is 1.62 mM in the presence of 10 mM substrates Escherichia coli
I303V/I65V/L186V/N104K product inhibition of the enzyme is reduced via semi-rational modification. The mutant enzyme shows a 3.3fold increase in specific activity when compared to wild-type enzyme. Specific acitivity of the mutant enzyme is 6.02 U/mg. Increased Ki(ATP) means reduced product inhibition which enhances accumulation of S-adenosyl-L-methionine. The S-adenosyl-L-methionine produced by the variant could reach to 2.68 mM while S-adenosyl-L-methionine produced by wild-type enzyme is 1.62 mM in the presence of 10 mM substrates Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
S-adenosyl-L-methionine product inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 ATP pH 8, 37°C, mutant enzyme I303V/I65V/L186V Escherichia coli
0.01
-
 ATP pH 8, 37°C, mutant enzyme I303V/I65V/L186V/N104K Escherichia coli
0.05
-
 ATP pH 8, 37°C, mutant enzyme I303V/I65V Escherichia coli
0.08
-
 L-methionine pH 8, 37°C, wild-type enzyme Escherichia coli
0.1
-
 ATP pH 8, 37°C, mutant enzyme I303V Escherichia coli
0.17
-
 ATP pH 8, 37°C, wild-type enzyme Escherichia coli
0.17
-
 L-methionine pH 8, 37°C, mutant enzyme I303V/I65V/L186V/N104K Escherichia coli
0.3
-
 L-methionine pH 8, 37°C, mutant enzyme I303V/I65V/L186V Escherichia coli
0.42
-
 L-methionine pH 8, 37°C, mutant enzyme I303V Escherichia coli
0.43
-
 L-methionine pH 8, 37°C, mutant enzyme I303V/I65V Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + H2O Escherichia coli
-
 phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?
ATP + L-methionine + H2O Escherichia coli K12
-
 phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A817
-
-
Escherichia coli K12 P0A817
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.82
-
 pH 8, 37°C, wild-type enzyme Escherichia coli
3.78
-
 pH 8, 37°C, mutant enzyme I303V/I65V/L186V Escherichia coli
6.02
-
 pH 8, 37°C, mutant enzyme I303V/I65V/L186V/N104K Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O
-
 Escherichia coli phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?
ATP + L-methionine + H2O
-
 Escherichia coli K12 phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?

Synonyms

Synonyms Comment Organism
MAT
-
 Escherichia coli
S-adenosylmethionine synthetase
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.22
-
 ATP pH 8, 37°C, wild-type enzyme Escherichia coli
1.22
-
 L-methionine pH 8, 37°C, wild-type enzyme Escherichia coli
1.3
-
 ATP pH 8, 37°C, mutant enzyme I303V Escherichia coli
1.3
-
 L-methionine pH 8, 37°C, mutant enzyme I303V Escherichia coli
1.94
-
 ATP pH 8, 37°C, mutant enzyme I303V/I65V Escherichia coli
1.94
-
 L-methionine pH 8, 37°C, mutant enzyme I303V/I65V Escherichia coli
3.28
-
 ATP pH 8, 37°C, mutant enzyme I303V/I65V/L186V Escherichia coli
3.28
-
 L-methionine pH 8, 37°C, mutant enzyme I303V/I65V/L186V Escherichia coli
4.34
-
 ATP pH 8, 37°C, mutant enzyme I303V/I65V/L186 V/N104K Escherichia coli
4.34
-
 L-methionine pH 8, 37°C, mutant enzyme I303V/I65V/L186V/N104K Escherichia coli