Literature summary for 2.5.1.6 extracted from

Liu, Y.; Wang, W.; Zhang, W.; Dong, Y.; Han, F.; Raza, M.; Liu, L.; Tan, T.; Feng, Y.
Structure of a thermostable methionine adenosyltransferase from: Thermus thermophilus HB27 reveals a novel fold of the flexible loop (2016), RSC Adv., 6, 41743-41750.

No PubMed abstract available

Search for more literature for 2.5.1.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 0.01 M iron-(III)-chloride hexahydrate, 0.1 M sodium citrate tribasic dehydrate pH 5.6 and 10% (v/v) Jeffamine M-600	Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42000	-	2 * 42000, SDS-PAGE	Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-methionine + H2O	Thermus thermophilus	-	phosphate + diphosphate + S-adenosyl-L-methionine	-	?
ATP + L-methionine + H2O	Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039	-	phosphate + diphosphate + S-adenosyl-L-methionine	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	Q72I53	-	-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039	Q72I53	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, and Superdex-200 gel filtration	Thermus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-methionine + H2O	-	Thermus thermophilus	phosphate + diphosphate + S-adenosyl-L-methionine	-	?
ATP + L-methionine + H2O	-	Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039	phosphate + diphosphate + S-adenosyl-L-methionine	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 42000, SDS-PAGE	Thermus thermophilus

Synonyms

Synonyms	Comment	Organism
MAT	-	Thermus thermophilus
methionine adenosyltransferase	-	Thermus thermophilus
S-adenosylmethionine synthase	-	Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	60	the enzyme activity is unchanged between 40 and 60°C after 24 h, while at 80°C, the enzyme activity drops to zero in 15 min	Thermus thermophilus

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Release 2023.1 (January 2023)