Cloned (Comment) | Organism |
---|---|
expression in Escheriochia coli | Pyrococcus furiosus |
General Stability | Organism |
---|---|
limited proteolysis experiments indicate that the proteolytic cleavage site is localized at Lys148 and that the C-terminal peptide is necessary for the integrity of the active site | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
L-methionine | pH 8.0, 80°C | Pyrococcus furiosus | |
0.115 | - |
ATP | pH 8.0, 80°C | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolutely dependent upon the presence of Mg2+, suggesting that a Mg2+-ATP complex functions as substrate. Maximal activity is obtained at a Mg2+ concentration of 20 mM | Pyrococcus furiosus | |
additional information | no stimulatory effect by K+ has been observed even at high concentration (40 mM) | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44279 | - |
2 * 44279, calculated from sequence | Pyrococcus furiosus |
45000 | - |
2 * 45000, SDS-PAGE | Pyrococcus furiosus |
90000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | Pyrococcus furiosus | the enzyme plays a key role in the biogenesis of S-adenosyl-L-methionine | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
guanidine-HCl | midpoint transition is 3.2 M | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8TZW1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.3 | - |
pH 8.0, 80°C | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Pyrococcus furiosus | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ATP + L-methionine + H2O | the enzyme plays a key role in the biogenesis of S-adenosyl-L-methionine | Pyrococcus furiosus | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 45000, SDS-PAGE | Pyrococcus furiosus |
homodimer | 2 * 44279, calculated from sequence | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
PF1866 | - |
Pyrococcus furiosus |
PfMAT | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 105 | 70°C: about 55% of maximal activity, 105°C: about 60% of maximal activity | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
1 h, stable | Pyrococcus furiosus |
80 | - |
1 h, 20% loss of activity | Pyrococcus furiosus |
95 | - |
half-life: 23 min | Pyrococcus furiosus |
99 | - |
Tm-value | Pyrococcus furiosus |
100 | - |
half-life: 5 min | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.5 | - |
ATP | pH 8.0, 80°C | Pyrococcus furiosus | |
7.5 | - |
L-methionine | pH 8.0, 80°C | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | half of the maximal activity at pH 7 and 9 | Pyrococcus furiosus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pyrococcus furiosus | calculated from sequence | - |
5.52 |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme plays a key role in the biogenesis of S-adenosyl-L-methionine | Pyrococcus furiosus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
65.2 | - |
ATP | pH 8.0, 80°C | Pyrococcus furiosus | |
242 | - |
L-methionine | pH 8.0, 80°C | Pyrococcus furiosus |