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Literature summary for 2.5.1.6 extracted from

  • Graham, D.E.; Bock, C.L.; Schalk-Hihi, C.; Lu, Z.J.; Markham, G.D.
    Identification of a highly diverged class of S-adenosylmethionine synthetases in the archaea (2000), J. Biol. Chem., 275, 4055-4059.
    View publication on PubMed
Search for more literature for 2.5.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 L-methionine pH 8.0, 58°C Methanocaldococcus jannaschii
0.25
-
 ATP pH 8.0, 58°C Methanocaldococcus jannaschii
1.4
-
 ITP pH 8.0, 58°C Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
K+ half-maximal activation at 25 mM KCl Methanocaldococcus jannaschii
Mg2+ no activity is detectable in the absence of Mg2+, and maximal activity is observed at 10 mM MgCl2 when ATP is present at 5 mM, indicating that MgATP is probably the true substrate Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + H2O Methanocaldococcus jannaschii biosynthesis of S-adenosyl-L-methionine phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?
ATP + L-methionine + H2O Methanocaldococcus jannaschii DSM 2661 biosynthesis of S-adenosyl-L-methionine phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58605
-
-
Methanocaldococcus jannaschii DSM 2661 Q58605
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O biosynthesis of S-adenosyl-L-methionine Methanocaldococcus jannaschii phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?
ATP + L-methionine + H2O two-step reaction in which reaction of ATP and methionine initially yields the S-adenosyl-L-methionine and tripolyphosphate. The tripolyphosphate remains enzyme-bound and is cleaved to diphosphate and phosphate before product release Methanocaldococcus jannaschii phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?
ATP + L-methionine + H2O biosynthesis of S-adenosyl-L-methionine Methanocaldococcus jannaschii DSM 2661 phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?
ATP + L-methionine + H2O two-step reaction in which reaction of ATP and methionine initially yields the S-adenosyl-L-methionine and tripolyphosphate. The tripolyphosphate remains enzyme-bound and is cleaved to diphosphate and phosphate before product release Methanocaldococcus jannaschii DSM 2661 phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?
ITP + L-methionine + H2O
-
 Methanocaldococcus jannaschii ?
-
 ?
ITP + L-methionine + H2O
-
 Methanocaldococcus jannaschii DSM 2661 ?
-
 ?

Synonyms

Synonyms Comment Organism
MAT
-
 Methanocaldococcus jannaschii
MJ1208 locus name Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
58
-
 assay at Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Methanocaldococcus jannaschii

General Information

General Information Comment Organism
physiological function the enzyme is involved in biosynthesis of S-adenosyl-L-methionine Methanocaldococcus jannaschii