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Literature summary for 2.5.1.6 extracted from

  • Luo, Y.; Yuan, Z.; Luo, G.; Zhao, F.
    Expression of secreted His-tagged S-adenosylmethionine synthetase in the methylotrophic yeast Pichia pastoris and its characterization, one-step purification, and immobilization (2008), Biotechnol. Prog., 24, 214-220.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Saccharomyces cerevisiae

General Stability

General Stability Organism
the Ni2+-IDA agarose-immobilized synthetase exhibits 40.4% of the free enzyme activity Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
AMP causes complete inactivation of the enzyme Saccharomyces cerevisiae
ATP causes complete inactivation of the enzyme Saccharomyces cerevisiae
Ba2+ 70.70% residual activity at 5 mM Saccharomyces cerevisiae
Br- 93.33% residual activity at 5 mM Saccharomyces cerevisiae
Ca2+ 86.36% residual activity at 5 mM Saccharomyces cerevisiae
CH3COO- 92.25% residual activity at 5 mM Saccharomyces cerevisiae
Cl- 85.27% residual activity at 5 mM Saccharomyces cerevisiae
Cu2+ 25.74% residual activity at 5 mM Saccharomyces cerevisiae
F- 88.84% residual activity at 5 mM Saccharomyces cerevisiae
Fe2+ 59.22% residual activity at 5 mM Saccharomyces cerevisiae
GSH causes complete inactivation of the enzyme Saccharomyces cerevisiae
I- 87.91% residual activity at 5 mM Saccharomyces cerevisiae
K+ 85.27% residual activity at 5 mM Saccharomyces cerevisiae
Li+ 81.40% residual activity at 5 mM Saccharomyces cerevisiae
Mn2+ 73.49% residual activity at 5 mM Saccharomyces cerevisiae
additional information no effect on activity at 0.1 mM Ni2+ Saccharomyces cerevisiae
Na+ 80.16% residual activity at 5 mM Saccharomyces cerevisiae
Zn2+ 22.17% residual activity at 5 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.527
-
L-methionine at pH 8.0 and 35°C Saccharomyces cerevisiae
0.631
-
ATP at pH 8.0 and 35°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+, H2PO4-, NH4-, and NO3- have no significant effect on enzyme activity at 5 mM Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P19358
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-IDA agarose column chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.42
-
crude extract, at 35°C Saccharomyces cerevisiae
23.83
-
after 3.71fold purification, at 35°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O
-
Saccharomyces cerevisiae phosphate + diphosphate + S-adenosyl-L-methionine
-
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Synonyms

Synonyms Comment Organism
ATP: L-methionine S-adenosyltransferase
-
Saccharomyces cerevisiae
S-adenosylmethionine synthetase
-
Saccharomyces cerevisiae
SAM synthetase
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 40 enzyme activity is significantly reduced at temperatures below 30°C or above 40°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 9 the activity of the enzyme is decreased above pH 9.0 and is inactive at pH below 7.5 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Saccharomyces cerevisiae