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Literature summary for 2.5.1.6 extracted from
- Role of an intrasubunit disulfide in the association state of the cytosolic homo-oligomer methionine adenosyltransferase (2003), J. Biol. Chem., 278, 7285-7293.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C35S | reduction in Vmax value | Rattus norvegicus |
| C61S | reduction in Vmax value | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.222 | - |
methionine | mutant C35S, presence of dithiothreitol | Rattus norvegicus |  |
| 0.246 | - |
methionine | wild-type, presence of dithiothreitol | Rattus norvegicus | |
| 0.449 | - |
methionine | mutant C35S, presence of glutathione | Rattus norvegicus | |
| 0.493 | - |
ATP | mutant C35S, presence of dithiothreitol | Rattus norvegicus | |
| 0.53 | - |
ATP | wild-type, presence of glutathione | Rattus norvegicus | |
| 0.588 | - |
ATP | wild-type, presence of dithiothreitol | Rattus norvegicus | |
| 0.756 | - |
methionine | mutant C61S, presence of dithiothreitol | Rattus norvegicus | |
| 0.778 | - |
ATP | mutant C35S, presence of glutathione | Rattus norvegicus | |
| 0.794 | - |
methionine | mutant C61S, presence of glutathione | Rattus norvegicus | |
| 1.12 | - |
methionine | wild-type, presence of glutathione | Rattus norvegicus | |
| 2.54 | - |
ATP | mutant C61S, presence of glutathione | Rattus norvegicus | |
| 3.25 | - |
ATP | mutant C61S, presence of dithiothreitol | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44300 | - |
2 * 44300, analysis of association/dissociation between dimer and tetramer by gel filtration and MALDI-TOF MS | Rattus norvegicus |
| 44300 | - |
4 * 44300, analysis of association/dissociation between dimer and tetramer by gel filtration and MALDI-TOF MS | Rattus norvegicus |
| 92700 | - |
gel filtration | Rattus norvegicus |
| 194900 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | P13444 | recombinant enzyme, isoform MAT I, tetramer, and MAT III, dimer | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 31500 | - |
mutant C61S, presence of glutathione | Rattus norvegicus |
| 32700 | - |
mutant C35S, presence of glutathione | Rattus norvegicus |
| 69070 | - |
mutant C61S, presence of dithiothreitol | Rattus norvegicus |
| 94470 | - |
mutant C35S, presence of dithiothreitol | Rattus norvegicus |
| 95800 | - |
wild-type, presence of glutathione | Rattus norvegicus |
| 110000 | - |
wild-type, presence of dithiothreitol | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + H2O | - |
Rattus norvegicus | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 44300, analysis of association/dissociation between dimer and tetramer by gel filtration and MALDI-TOF MS | Rattus norvegicus |
| tetramer | 4 * 44300, analysis of association/dissociation between dimer and tetramer by gel filtration and MALDI-TOF MS | Rattus norvegicus |
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