Literature summary for 2.5.1.6 extracted from

Perez-Pertejo, Y.; Reguera, R.M.; Villa, H.; Garcia-Estrada, C.; Balana-Fouce, R.; Pajares, M.A.; Ordonez, D.
Leishmania donovani methionine adenosyltransferase. Role of cysteine residues in the recombinant enzyme (2003), Eur. J. Biochem., 270, 28-35.

Search for more literature for 2.5.1.6

Activating Compound

Activating Compound	Comment	Organism	Structure
S-adenosylmethionine	nonessential activator of tripolyphosphatase activity in the range of 0.005-0.100 mM	Leishmania donovani

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli XL-1Blue strain	Leishmania donovani

Inhibitors

Inhibitors	Comment	Organism	Structure
N-ethylmaleimide	time-dependent inactivation of both MAT activities	Leishmania donovani
S-adenosylmethionine	non competitive inhibition	Leishmania donovani

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	MAT II, gel filtration	Leishmania donovani

Organism

Organism	UniProt	Comment	Textmining
Leishmania donovani	Q9U6Q8	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
refolding by a fourfold dilution refolding buffer up to a concentration of 2 M urea	Leishmania donovani

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.058	-	tripolyphosphase activity	Leishmania donovani
0.2	-	S-adenosylmethionine synthesis activity	Leishmania donovani

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-methionine + H2O	-	Leishmania donovani	phosphate + diphosphate + S-adenosyl-L-methionine	-	?
tripolyphosphate + H2O	-	Leishmania donovani	diphosphate + phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Leishmania donovani

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.004	-	tripolyphosphate	tripolyphosphase activity	Leishmania donovani
0.032	-	S-adenosylmethionine	S-adenosylmethionine synthesis activity	Leishmania donovani

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.8	-	S-adenosylmethionine	ATP concentrations of 0.5-5 mM	Leishmania donovani
4	-	S-adenosylmethionine	L-methionine concentrations of 0.5-5 mM	Leishmania donovani

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)