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Literature summary for 2.5.1.6 extracted from

  • Lopez-Vara, M.C.; Gasset, M.; Pajares, M.A.
    Refolding and characterization of rat liver methionine adenosyltransferase from Escherichia coli inclusion bodies (2000), Protein Expr. Purif., 19, 219-226.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
monomer, gel filtration and immunoblotting Rattus norvegicus
89130
-
glutathione/glutathione disulfide-refolded MAT III, gel filtration Rattus norvegicus
92700
-
dithiothreitol-refolded MAT III, gel filtration Rattus norvegicus
110000
-
MAT III, gel filtration Rattus norvegicus
194900
-
glutathione/glutathione disulfide-refolded MAT I, gel filtration Rattus norvegicus
210000
-
MAT I Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
method that includes DEAE-Sephacel chromatography and phenyl Sepharose CL-4B chromatography Rattus norvegicus

Renatured (Commentary)

Renatured (Comment) Organism
refolding by direct dialysis or by dilution after treatment with urea under several conditions Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
different values in refolding and purification process Rattus norvegicus
0.039
-
MAT I Rattus norvegicus
0.096
-
glutathione/glutathione disulfide-refolded MAT III Rattus norvegicus
0.098
-
MAT III Rattus norvegicus
0.1
-
glutathione/glutathione disulfide-refolded MAT I Rattus norvegicus
0.11
-
dithiothreitol-refolded MAT III Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9.5 glutathione/glutathione disulfide-refolded MAT I Rattus norvegicus
7.7 8.8 MAT III, MAT I and dithiothreitol-refolded MAT III Rattus norvegicus
8.5 10 glutathione/glutathione disulfide-refolded MAT III Rattus norvegicus