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Literature summary for 2.5.1.6 extracted from
- Cloning, expression, and functional characterization of the b regulatory subunit of human methionine adenosyltransferase (MAT II) (2000), J. Biol. Chem., 275, 2359-2366.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli M15(Qiagen) | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
L-methionine | one kinetic form of alpha-two subunit in the presence of beta subunit | Homo sapiens |  |
| 0.03 | 0.038 | L-methionine | one kinetic form of alpha subunit in the presence of beta subunit | Escherichia coli | |
| 0.06 | 0.1 | L-methionine | alpha-two subunit | Homo sapiens | |
| 0.065 | 0.08 | L-methionine | alpha subunit at low L-methionine concentrations | Escherichia coli | |
| 0.076 | - |
L-methionine | one kinetic form of alpha-two subunit in the presence of beta subunit | Homo sapiens | |
| 0.08 | 0.09 | L-methionine | alpha subunit at high L-methionine concentrations. Also one kinetic form of alpha subunit in the presence of beta subunit | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | 39000 | MAT II beta subunit, gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Homo sapiens | Q9NZL9 | MAT-II | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| two methods: the first involved purification of the His-tagged protein under denaturing conditions of 8M urea and the second involved separation of SDS-PAGE | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + H2O | - |
Escherichia coli | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
| ATP + L-methionine + H2O | - |
Homo sapiens | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | - |
Homo sapiens |
| More | beta subunit has a regulatory function. MAT II beta subunit associates spontaneously with Escherichia coli MAT alpha subunit as well as with the recombinant human MAT II alpha-two subunit | Homo sapiens |
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