Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
Dimethylsulfoxide | - |
Homo sapiens | |
diphosphate | - |
Homo sapiens | |
L-methionine | - |
Homo sapiens | |
additional information | overview of the regulatory properties, effect of L-methionine analogues and influence of L-methionine concentration on activating and inhibiting effects, effect of tripolyphosphate and p-hydroxymercuribenzoate | Homo sapiens | |
p-chloromercuribenzoate | - |
Homo sapiens | |
phosphate | - |
Homo sapiens | |
S-adenosylmethionine | non competitive with ATP at low methionine concentration | Homo sapiens | |
tripolyphosphate | competitive with ATP and non competitive with L-methionine | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
ATP | - |
Homo sapiens | |
3.3 | - |
L-methionine | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ in excess of that bound to ATP and EDTA is required for optimal activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
185000 | - |
equilibrium sedimentation studies | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine | steady state ordered Bi Ter mechanism with ATP adding before L-methionine and S-adenosylmethionine being the first product released, random release of phosphate and diphosphate | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lymphocyte | from chronic leukemia cells | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
12.2 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-70°C, less than 10% loss of activity after 3 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxy-ATP + L-methionine + H2O | - |
Homo sapiens | ? | - |
? | |
3'-deoxy-ATP + L-methionine + H2O | - |
Homo sapiens | ? | - |
? | |
ATP + L-methionine + H2O | - |
Homo sapiens | S-adenosyl-L-methionine + phosphate + diphosphate | - |
? | |
tripolyphosphate + H2O | tripolyphosphatase activity | Homo sapiens | diphosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Homo sapiens |
tetramer | heterotetramer with subunit composition alpha-alpha'-beta2, alpha2beta2 or alpha'2beta2, where alpha, alpha', and beta are chains of molecular weight 53000, 51000 and 38000 | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
stable for several hours, if protein concentration is above 0.1 mg/ml and ionic strength is at least 50 mM | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Homo sapiens |