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Literature summary for 2.5.1.6 extracted from

  • Markham, G.D.; Hafner, E.W.; White Tabor, C.; Tabor, H.
    S-adenosylmethionine synthetase (methionine adenosyltransferase) (Escherichia coli) (1983), Methods Enzymol., 94, 219-222.
    View publication on PubMed
Search for more literature for 2.5.1.6

Inhibitors

Inhibitors Comment Organism Structure
Adenyl-5'-ylimidodiphosphate competitive with ATP Escherichia coli
diphosphate
-
 Escherichia coli
phosphate
-
 Escherichia coli
S-adenosylmethionine
-
 Escherichia coli
S-carbamylcysteine competitive with methionine Escherichia coli
tripolyphosphate
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ for maximal activation the cation concentration must be at least equal to ATP concentration Escherichia coli
Mg2+ cation concentration must be at least equal to ATP concentration for maximal activity Escherichia coli
Mn2+ for maximal activation the cation concentration must be at least equal to ATP concentration Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000 Escherichia coli
180000
-
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli
0.75
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-70°C, 5 mg/ml enzyme concentration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxy-ATP + L-methionine + H2O
-
 Escherichia coli ?
-
 ?
3'-deoxy-ATP + L-methionine + H2O
-
 Escherichia coli ?
-
 ?
ATP + L-methionine + H2O
-
 Escherichia coli S-adenosyl-L-methionine + phosphate + diphosphate
-
 ?
additional information ATP can be substituted by 3'-deoxy-ATP,8-bromo-ATP, formycin triphosphate, adenyl-5'yl imidodiphosphate Escherichia coli ?
-
 ?
tripolyphosphate + H2O tripolyphosphatase activity Escherichia coli diphosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 43000 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli