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Literature summary for 2.5.1.59 extracted from

  • Boutin, J.A.; Marande, W.; Goussard, M.; Loynel, A.; Canet, E.; Fauchere, J.L.
    Chromatographic assay and peptide substrate characterization of partially purified farnesyl- and geranylgeranyltransferases from rat brain cytosol (1998), Arch. Biochem. Biophys., 354, 83-94.
    View publication on PubMed

Application

Application Comment Organism
medicine S-prenylated enzymes are interesting targets for noncytotoxic anticancer agents Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information comparison of Km of 18 hexapeptides as potential substrates of the farnesyltransferase and geranylgeranyl transferaseI, recognition of overlapping sequences by farnesyltransferase and geranylgeranyltransferaseI Rattus norvegicus
0.00002
-
Arg-Arg-Cys-Val-Leu-Leu pH 7.6, 30°C Rattus norvegicus
0.00013
-
Lys-Lys-Cys-Ile-Ile-Met pH 7.6, 30°C Rattus norvegicus
0.002
-
Lys-Pro-Cys-Val-Val-Met pH 7.6, 30°C Rattus norvegicus
0.002
-
Phe-Phe-Cys-Ala-Ile-Leu pH 7.6, 30°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate this enzyme, along with protein farnesyltransferase, EC 2.5.1.58 and protein geranylgeranyltransferase type II, EC 2.5.1.60, constitutes the protein prenyltransferase family of enzymes Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate + Arg-Arg-Cys-Val-Leu-Leu
-
Rattus norvegicus diphosphate + Arg-Arg-S-geranylgeranyl-Cys-Val-Leu-Leu
-
?
geranylgeranyl diphosphate + Leu-Pro-Cys-Val-Val-Met
-
Rattus norvegicus diphosphate + Leu-Pro-S-geranylgeranyl-Cys-Val-Val-Met
-
?
geranylgeranyl diphosphate + Lys-Lys-Cys-Ile-Ile-Met
-
Rattus norvegicus diphosphate + Lys-Lys-S-geranylgeranyl-Cys-Ile-Ile-Met
-
?
geranylgeranyl diphosphate + Lys-Pro-Cys-Val-Val-Met
-
Rattus norvegicus Lys-Pro-(S-geranylgeranyl)-Cys-Val-Val-Met + diphosphate
-
?
geranylgeranyl diphosphate + Phe-Phe-Cys-Ala-Ile-Leu
-
Rattus norvegicus diphosphate + Phe-Phe-S-geranylgeranyl-Cys-Ala-Ile-Leu
-
?
geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X Rattus norvegicus S-geranylgeranyl-protein + diphosphate
-
?
additional information some of the 18 analysed hexapeptides effieciently farnsylated by enzyme Rattus norvegicus ?
-
?