Literature summary for 2.5.1.59 extracted from

Ohya, Y.; Caplin, B.E.; Qadota, H.; Tibbetts, M.F.; Anraku, Y.; Pringle, J.R.; Marshall, M.S.
Mutational analysis of the beta-subunit of yeast geranylgeranyl transferase I (1996), Mol. Gen. Genet., 252, 1-10.

Search for more literature for 2.5.1.59

Cloned(Commentary)

Cloned (Comment)	Organism
wild-type and mutant enzymes expressed in Escherichia coli	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
additional information	cal1-1 mutation preferentially affects Rho1p geranylgeranylation, and it can be suppressed by Rho1p overexpression, the cdc43-5 mutation in the same gene causes accumulation of Cdc42p in soluble form and can be suppressed by Cdc42p overproduction	Saccharomyces cerevisiae
additional information	seven different mutations in CAL1/CDC43 gene: cal1-1 and cdc43-2 to cdc43-7, all of mutants possess reduced activity and none show temperature-sensitive enzymatic activities, but all of them show temperature-sensitive growth phenotypes	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
geranylgeranyl diphosphate + protein-cysteine	Saccharomyces cerevisiae	this prenylation is necessary for many proteins to interact with membrane localized at proper intracellular sites	S-geranylgeranyl-protein + diphosphate	-	?
geranylgeranyl diphosphate + protein-cysteine	Saccharomyces cerevisiae	yeast enzyme is essential for yeast cell growth	S-geranylgeranyl-protein + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	strains YOT433-2C, YOT434-2B, YOT435-1A, YOT436-3C, YOT437-2C	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant enzymes	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranylgeranyl diphosphate + protein-cysteine	prenylation, substrates are Rho, Rac, most trimeric G protein gamma subunits	Saccharomyces cerevisiae	S-geranylgeranyl-protein + diphosphate	-	?
geranylgeranyl diphosphate + protein-cysteine	this prenylation is necessary for many proteins to interact with membrane localized at proper intracellular sites	Saccharomyces cerevisiae	S-geranylgeranyl-protein + diphosphate	-	?
geranylgeranyl diphosphate + protein-cysteine	yeast enzyme is essential for yeast cell growth	Saccharomyces cerevisiae	S-geranylgeranyl-protein + diphosphate	-	?

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)