KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
Ras-Cys-Val-Leu-Leu | pH 7.5, 37°C | Homo sapiens | |
0.0021 | - |
N-Ras | pH 7.5, 37°C | Homo sapiens | |
0.0088 | - |
Ki-Ras4A | pH 7.5, 37°C | Homo sapiens | |
0.012 | - |
Ki-Ras4B | pH 7.5, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
enzyme produced in Sf9cells using baculovirus system | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + Ki-Ras4B | - |
Homo sapiens | diphosphate + S-geranylgeranyl-Ki-Ras4B | - |
? | |
geranylgeranyl diphosphate + Ki-Ras4A | - |
Homo sapiens | diphosphate + S-geranylgeranyl-Ki-Ras4A | - |
? | |
geranylgeranyl diphosphate + N-Ras | - |
Homo sapiens | diphosphate + S-geranylgeranyl-N-Ras | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-Leu | Homo sapiens | S-geranylgeranyl-protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + protein-cysteine | enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X | Homo sapiens | S-geranylgeranyl-protein + diphosphate | - |
? | |
geranylgeranyl diphosphate + Ras-Cys-Val-Leu-Leu | - |
Homo sapiens | diphosphate + Ras-S-geranylgeranyl-Cys-Val-Leu-Leu | - |
? | |
geranylgeranyl diphosphate + S-geranylgeranyl-Ki-Ras4B | both the polylysine and the carboxy-terminal methionine are important for geranylgeranylation of this substrate | Homo sapiens | diphosphate + S-geranylgeranyl-Ki-Ras4B | - |
? | |
additional information | not: N-Ras | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0113 | - |
N-Ras | - |
Homo sapiens | |
0.014 | - |
Ras-Cys-Val-Leu-Leu | - |
Homo sapiens | |
0.0667 | - |
Ki-Ras4A | - |
Homo sapiens | |
0.0767 | - |
Ki-Ras4B | - |
Homo sapiens |