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Literature summary for 2.5.1.59 extracted from

  • Yokoyama, K.; Gelb, M.H.
    Purification of a mammalian protein geranylgeranyltransferase. Formation and catalytic properties of an enzyme-geranylgeranyl pyrophosphate complex (1993), J. Biol. Chem., 268, 4055-4060.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
Ser-Ser-Cys-Ile-Leu-Leu pH 7.4, 30°C Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
1 * 48000 + 1 * 40000 Bos taurus
48000
-
1 * 48000 + 1 * 40000 Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate + protein-cysteine Bos taurus
-
S-geranylgeranyl-protein + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
peptide affinity chromatography of enzyme on SSCILL-Sepharose Bos taurus

Reaction

Reaction Comment Organism Reaction ID
geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate enzyme and geranylgeranyl diphosphate produce a stable binary complex and the tightly bound geranylgeranyl diphosphate is transferred to a peptide acceptor, without the equilibration of enzyme-bound geranylgeranyl diphosphate with free geranylgeranyl diphosphate Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0059
-
-
Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu ECB-gamma6 Bos taurus diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFF-S-farnesyl-CAI-Leu
-
?
geranylgeranyl diphosphate + (biotin-CONH-(CH2)5-CO-)-ALEPPETEPKRKCCIF ECB-G25K, 42% activity in comparison of protein substrate motif: CAA-leucine Bos taurus diphosphate + (biotin-CONH-(CH2)5-CO-)-ALEPPETEPKRK-S-geranylgeranyl-CCIF
-
?
geranylgeranyl diphosphate + (biotin-CONH-(CH2)5-CO-)-GTPRASNRSCAIS ECB-laminB(S), 5% activity in comparison of protein substrate motif: CAA-leucine Bos taurus diphosphate + (biotin-CONH-(CH2)5-CO-)-GTPRASNRS-S-geranylgeranyl-CAIS
-
?
geranylgeranyl diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFFCAI-Leu ECB-gamma6, preferred substrate Bos taurus diphosphate + (biotin-CONH-(CH2)5-CO-)-NPFREKKFF-S-geranylgeranyl-CAI-Leu
-
?
geranylgeranyl diphosphate + protein-cysteine
-
Bos taurus S-geranylgeranyl-protein + diphosphate
-
?
geranylgeranyl diphosphate + protein-cysteine enzyme requires that protein substrates contain a Cys residue fourth from the C terminus, protein substrate motif: Cys-aliphatic-aliphatic-X. X is Leu, Phe or Ser, preferred substrate: Leu Bos taurus S-geranylgeranyl-protein + diphosphate
-
?
Ser-Ser-Cys-Ile-Leu-Leu + geranylgeranyl diphosphate
-
Bos taurus diphosphate + Ser-Ser-S-geranylgeranyl-Cys-Ile-Leu-Leu
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 48000 + 1 * 40000 Bos taurus