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Literature summary for 2.5.1.58 extracted from

  • Kumagai, M.; Makioka, A.; Takeuchi, T.; Nozaki, T.
    Molecular cloning and characterization of a protein farnesyltransferase from the enteric protozoan parasite Entamoeba histolytica (2004), J. Biol. Chem., 279, 2316-2323.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
recombinant alpha- and beta-subunits, coexpressed in Escherichia coli, form a heterodimer and show activity to transfer farnesyl using farnesylpyrophosphate as a donor to human H-Ras possessing a C-terminal CVLS, but not a mutant H-Ras possessing CVLL. Entamoeba histolytica

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10 mM Entamoeba histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35600
-
 1 * 35600 + 1 * 42600, calculation from nucleotide sequence Entamoeba histolytica
38000
-
 1 * 38000 + 1 * 43000, SDS-PAGE Entamoeba histolytica
42600
-
 1 * 35600 + 1 * 42600, calculation from nucleotide sequence Entamoeba histolytica
43000
-
 1 * 38000 + 1 * 43000, SDS-PAGE Entamoeba histolytica
80000
-
 gel filtration Entamoeba histolytica

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica Q76L89 subunit Ftbeta
-
Entamoeba histolytica Q76L90 subunit Ftalpha
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl diphosphate + human recombinant wild-type H-Ras-CVLS
-
 Entamoeba histolytica diphosphate + ?
-
 ?
farnesyl diphosphate + mutant H-Ras-CVLL 20fold less active than human recombinant wild-type H-Ras-CVLS Entamoeba histolytica diphosphate + ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 35600 + 1 * 42600, calculation from nucleotide sequence Entamoeba histolytica
dimer 1 * 38000 + 1 * 43000, SDS-PAGE Entamoeba histolytica

Synonyms

Synonyms Comment Organism
EhFT
-
 Entamoeba histolytica