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Literature summary for 2.5.1.58 extracted from

  • Saderholm, M.J.; Hightower, K.E.; Fierke, C.A.
    Role of metals in the reaction catalyzed by protein farnesyltransferase (2000), Biochemistry, 39, 12398-12405.
    View publication on PubMed

Application

Application Comment Organism
medicine
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Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the magnesium affinity of enzyme increases with pH with a pka of 7.4, presumably reflecting the deprotonation of the farnesyl diphosphate to enhance magnesium coordination Rattus norvegicus
Mg2+ appears to coordinate the diphosphate moiety of farnesyl diphosphate Rattus norvegicus
additional information a metal-assisted nucleophile is involved in the catalytic mechanism of enzyme Rattus norvegicus
Zn2+ required Rattus norvegicus
Zn2+ zinc metalloenzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
farnesyl diphosphate + protein-cysteine Rattus norvegicus process necessary for the subcellular localisation of substrate to the plasma membrane S-farnesyl protein + diphosphate
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?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
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-
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Reaction

Reaction Comment Organism Reaction ID
farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate kinetic mechanism Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl diphosphate + protein-cysteine prenylation, farnesylation, substrates are Ras, nuclear lamins, transducin gamma subunit, protein substrate motif: Cys-aliphatic-aliphatic-X, X: M, S, Q, A, F Rattus norvegicus diphosphate + S-farnesyl protein
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?
farnesyl diphosphate + protein-cysteine process necessary for the subcellular localisation of substrate to the plasma membrane Rattus norvegicus S-farnesyl protein + diphosphate
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?
farnesyl monophosphate + Gly-Cys-Val-Leu-Ser
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Rattus norvegicus phosphate + Gly-S-farnesyl-Cys-Val-Leu-Ser
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?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
pH-dependence of the chemical step of product formation: the reaction is characterized by two ionizations, drastic differences in the pH dependence of farnesyl diphophate and farnesyl monophosphate, the magnesium affinity of enzyme increases with pH with a pka of 7.4 Rattus norvegicus