Literature summary for 2.5.1.58 extracted from

Chen, W.J.; Moomaw, J.F.; Overton, L.; Kost, T.A.; Casey, P.J.
High level expression of mammalian protein farnesyltransferase in a baculovirus system (1993), J. Biol. Chem., 286, 9675-9680.

Search for more literature for 2.5.1.58

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	infection of Sf9 cells with the recombinant baculovirus containing DNA sequences encoding both enzyme activity results in a dramatic enhancement of enzyme activity	Rattus norvegicus

Cloned(Commentary)

Cloned (Comment)	Organism
coexpression of alpha and beta subunits of enzyme by Sf9 cells infected with a recombinant baculovirus	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
Cys-Val-Phe-Met	-	Rattus norvegicus
additional information	hexapeptide containing a switch of leucine for methionine at the COOH terminus or a switch of cysteine for serine at the prenylation site is essentially inactive in competing for enzyme activity	Rattus norvegicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	-	Rattus norvegicus
additional information	not: Ca2+	Rattus norvegicus
additional information	Co2+	Rattus norvegicus
Zn2+	zinc metalloenzyme	Rattus norvegicus
Zn2+	enzyme contains a stoichiometric amount of zinc	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	-	alpha,beta, 1 * 48000 + 1 * 46000	Rattus norvegicus
48000	-	alpha,beta, 1 * 48000 + 1 * 46000	Rattus norvegicus

Organic Solvent Stability

Organic Solvent	Comment	Organism
additional information	EDTA-treatment decreases activity	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
from recombinant baculovirus-infected Sf9 cells	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0085	-	-	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
farnesyl diphosphate + protein-cysteine	prenylation, farnesylation, substrates are Ras, nuclear lamins, transducin gamma subunit, protein substrate motif: Cys-aliphatic-aliphatic-X, X: M, S, Q, A, F	Rattus norvegicus	diphosphate + S-farnesyl protein	-	?

Subunits

Subunits	Comment	Organism
heterodimer	SDS-PAGE	Rattus norvegicus
heterodimer	alpha,beta, 1 * 48000 + 1 * 46000	Rattus norvegicus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)