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Literature summary for 2.5.1.57 extracted from

  • Reaves, M.L.; Lopez, L.C.; Daskalova, S.M.
    Replacement of the antifreeze-like domain of human N-acetylneuraminic acid phosphate synthase with the mouse antifreeze-like domain impacts both N-acetylneuraminic acid 9-phosphate synthase and 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthase activities (2008), BMB rep., 41, 72-78.
    View publication on PubMed
Search for more literature for 2.5.1.57

Protein Variants

Protein Variants Comment Organism
additional information by replacing the antifreeze-like domain of the human NeuNAc-9-P synthase with the mouse antifreeze-like domain, the chimeric protein retains almost half of the ability of the human enzyme for 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate synthesis while the N-acetylneuraminate 9-phosphate production is reduced to less than 10%, the chimeric enzyme is less resistant to high temperatures compared to the wild type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ 12.5 mM Mg2+ decrease the ability for N-acetylneuraminate 9-phosphate synthesis by almost half, while the 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate synthetic activity drops by 20% Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activity is favored by the presence of Mn2+ ions at a concentration of 12.5 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 2 * 41000, gel filtration Homo sapiens
43000
-
 2 * 43000, SDS-PAGE Homo sapiens
82000
-
 gel filtration Homo sapiens
86000
-
 SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NR45
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.22
-
 in the presence of 12.5 mM MnCl2, 8.3 mM phosphoenolpyruvate and 12.5 mM D-mannose 6-phosphate Homo sapiens
11.7
-
 in the presence of 12.5 mM MnCl2, 8.3 mM phosphoenolpyruvate and 2.5 mM N-acyl-D-mannosamine 6-phosphate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannose 6-phosphate + phosphoenolpyruvate + H2O
-
 Homo sapiens 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid 9-phosphate + phosphate
-
 ?
N-acyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
 Homo sapiens N-acylneuraminate 9-phosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 43000, SDS-PAGE Homo sapiens
homodimer 2 * 41000, gel filtration Homo sapiens

Synonyms

Synonyms Comment Organism
N-acetylneuraminic acid phosphate synthase
-
 Homo sapiens
NeuNAc phosphate synthase
-
 Homo sapiens
NeuNAc-9-P synthase
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 65 the enzyme loses 43% of its initial KDN-9-P synthetic activity after 10 min at 37°C or 55°C, treatment for 10 min at 65°C results in retention of 26% and 39% of N-acetylneuraminate 9-phosphate and 2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid-9-phosphate activity respectively Homo sapiens