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Literature summary for 2.5.1.57 extracted from

  • Chen, H.; Blume, A.; Zimmermann-Kordmann, M.; Reutter, W.; Hinderlich, S.
    Purification and characterization of N-acetylneuraminic acid-9-phosphate synthase from rat liver (2002), Glycobiology, 12, 65-71.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

General Stability

General Stability Organism
protected from inactivation by presence of phosphoenolpyruvate Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithio-bis(2-nitrobenzoic acid)
-
Rattus norvegicus
N-ethylmaleimide
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
N-acetyl-D-mannosamine 6-phosphate
-
Rattus norvegicus
0.1
-
phosphoenolpyruvate
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ absolute requirement for divalent cations Rattus norvegicus
Mg2+ absolute requirement for divalent cations Rattus norvegicus
Mn2+ absolute requirement for divalent cations Rattus norvegicus
additional information Ca2+ or Cu2+ does not substitute for Mg2+ Rattus norvegicus
Ni2+ absolute requirement for divalent cations Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
2 * 37000, SDS-PAGE Rattus norvegicus
75000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O Escherichia coli
-
N-acetyl-neuraminate 9-phosphate + phosphate
-
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O Rattus norvegicus
-
N-acetyl-neuraminate 9-phosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
neuB gene product amino acid sequence accession. no.
-
Homo sapiens Q9NR45 amino acid sequence
-
Rattus norvegicus
-
rat
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
salivary gland
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.505
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, crude extract retains 60% of enzyme activity when stored for 1 week Rattus norvegicus
4°C, purified enzyme loses activity completely after 1 day in phosphate buffer Rattus norvegicus
4°C, purified enzyme, 50% of enzyme activity remains after 2 days in phosphate buffer containing 5 mM MgCl2, enzyme activity is lost entirely after 3 days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
Escherichia coli N-acetyl-neuraminate 9-phosphate + phosphate
-
?
N-acetyl-D-mannosamine 6-phosphate + phosphoenolpyruvate + H2O
-
Rattus norvegicus N-acetyl-neuraminate 9-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 37000, SDS-PAGE Rattus norvegicus