Cloned (Comment) | Organism |
---|---|
gene kdsA1, in Arabidopsis a functionally redundant gene pair encodes Kdo-8-P synthases, AtKdsA1 (At1g9500) and AtKdsA2 (At1g16340), recombinant expression in Escherichia coli | Arabidopsis thaliana |
gene kdsA2, in Arabidopsis a functionally redundant gene pair encodes Kdo-8-P synthases, AtKdsA1 (At1g9500) and AtKdsA2 (At1g16340), recombinant expression in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | gene kdsA1 single knockout and kdsA1/kdsA2 double knockout mutant constructions | Arabidopsis thaliana |
additional information | gene kdsA2 single knockout and kdsA1/kdsA2 double knockout mutant constructions | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6H-6-Imino-(2,3,4,5-tetrahydropyrimido)[1,2-c]-[1,3]benzothiazine | PD404,182,displays strong inhibition in vitro, but is ineffective in vivo against Gram-negative bacteria due to an inability to cross the bacterial plasma membran | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | Escherichia coli | - |
3-deoxy-D-manno-octulosonate 8-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | Arabidopsis thaliana | - |
3-deoxy-D-manno-octulosonate 8-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q6NQL4 | gene kdsA2; gene kdsA2 | - |
Arabidopsis thaliana | Q9AV97 | gene kdsA1; gene kdsA1 | - |
Escherichia coli | - |
gene kdsA | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate | the catalytic mechanism is thought to occur by correct substrate proximity and orientation through a series of sequential conformational changes in which phosphoenolpyruvate binding precedes that of D-arabinose 5-phosphate and the release of inorganic phosphate (Pi) precedes dissociation of the 3-deoxy-D-manno-octulosonate 8-phosphate product | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | - |
Escherichia coli | 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-arabinose 5-phosphate + H2O | - |
Arabidopsis thaliana | 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | in solution | Arabidopsis thaliana |
tetramer | tetrameric quaternary structure with an active site in each monomer, by NMR study and crystal structure analysis | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
KDO-8-P synthase | - |
Escherichia coli |
KDO-8-P synthase | - |
Arabidopsis thaliana |
Kdo-8-phosphate synthase | - |
Escherichia coli |
Kdo-8-phosphate synthase | - |
Arabidopsis thaliana |
KDOPS | - |
Escherichia coli |
KDOPS | - |
Arabidopsis thaliana |
KdsA | - |
Arabidopsis thaliana |
KdsA | - |
Escherichia coli |
KdsA1 | - |
Arabidopsis thaliana |
KdsA2 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.9 | - |
phosphoenolpyruvate | pH and temperature not specified in the publication, recombinant enzyme | Arabidopsis thaliana | |
5.9 | - |
D-arabinose 5-phosphate | pH and temperature not specified in the publication, recombinant enzyme | Arabidopsis thaliana | |
6.8 | - |
phosphoenolpyruvate | pH and temperature not specified in the publication | Escherichia coli | |
6.8 | - |
D-arabinose 5-phosphate | pH and temperature not specified in the publication | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000026 | - |
6H-6-Imino-(2,3,4,5-tetrahydropyrimido)[1,2-c]-[1,3]benzothiazine | pH and temperature not specified in the publication | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | Arabidopsis thaliana kdsA male gametophyte with lethal phenotype | Arabidopsis thaliana |
malfunction | while single T-DNA mutants of either gene display no visible phenotypes, generation of a double knockout mutant has not been possible due to a failure of haploid pollen tube elongation | Arabidopsis thaliana |