Literature summary for 2.5.1.54 extracted from

Balachandran, N.; Heimhalt, M.; Liuni, P.; To, F.; Wilson, D.J.; Junop, M.S.; Berti, P.J.
Potent inhibition of 3-deoxy-D-arabinoheptulosonate-7-phosphate (DAHP) synthase by DAHP oxime, a phosphate group mimic (2016), Biochemistry, 55, 6617-6629 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with inhibitor 3-deoxy-D-arabinoheptulosonate-7-phosphate oxime	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
3-deoxy-D-arabinoheptulosonate-7-phosphate oxime	binding is competitive with respect to all three substrates.The oxime functional group, combined with two crystallographic waters, binds at the same location in the catalytic center as the phosphate group of the tetrahedral intermediate. DAHP oxime binds to only one subunit of each tight dimer	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.015	-	D-erythrose 4-phosphate	pH 7.5, 25°C	Escherichia coli
0.14	-	phosphoenolpyruvate	pH 7.5, 25°C	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	required, Km value 0.0055 mM for rapid equilibrium assumption	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	C3TIE2	isoform DAHPS(Phe)	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	kinetic mechanism is rapid equilibrium sequential ordered ter ter, with the essential divalent metal ion, Mn2+, binding first, followed by phosphoenolpyruvate and D-erythrose 4-phosphate	Escherichia coli	?	-	-
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O	-	Escherichia coli	3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
AroG	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
16.4	-	phosphoenolpyruvate	pH 7.5, 25°C	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0015	-	3-deoxy-D-arabinoheptulosonate-7-phosphate oxime	pH 7., 25°C	Escherichia coli

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Release 2023.1 (January 2023)