Literature summary for 2.5.1.54 extracted from

Zhao, H.; Gao, H.; Ji, K.; Yan, B.; Li, Q.; Mo, S.; Zheng, M.; Ou, Q.; Wu, B.; Li, N.; Jiang, C.
Isolation and biochemical characterization of a metagenome-derived 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase gene from subtropical marine mangrove wetland sediments (2019), AMB Express, 9, 19 .

Search for more literature for 2.5.1.54

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	uncultured bacterium

Crystallization (Commentary)

Crystallization (Comment)	Organism
molecular modeling of structure. The monomeric structure of Aro1A is a (beta/alpha)8 barrel structure. Phosphoenolpyruvate combines with residues R60, Q116, S119, K141, and R171 through eight hydrogen bonds. D-erythrose 4-phosphate combines with residues R60, K65, Q116, S119, K141, and R171 through nine hydrogen bonds	uncultured bacterium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0196	-	phosphoenolpyruvate	pH 8.0, 40°C	uncultured bacterium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ba2+	presence of Ba2+ stimulates the activity by more than 300%	uncultured bacterium
Ca2+	presence of Ca2+ stimulates the activity by more than 300%	uncultured bacterium
Mn2+	presence of Mn2+ stimulates the activity by more than 250%, Mn2+ is liganded by residues C36, H206, E232, and D243	uncultured bacterium
additional information	not stimulating: Zn2+, Mg2+	uncultured bacterium

Organism

Organism	UniProt	Comment	Textmining
uncultured bacterium	A0A482F152	metagenomic library from subtropical marine mangrove sediment	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O	-	uncultured bacterium	3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 37000, SDS-PAGE	uncultured bacterium

Synonyms

Synonyms	Comment	Organism
Aro1A	-	uncultured bacterium
Dax1	-	uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	uncultured bacterium

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	47	more than 60% of maximum activity	uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
17.3	-	phosphoenolpyruvate	pH 8.0, 40°C	uncultured bacterium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	uncultured bacterium

pH Range

pH Minimum	pH Maximum	Comment	Organism
7.3	8.5	more than 60% of maximum activity	uncultured bacterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
880	-	phosphoenolpyruvate	pH 8.0, 40°C	uncultured bacterium

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)