Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aeropyrum pernix |
Crystallization (Comment) | Organism |
---|---|
in complex with a manganese ion and phosphoenolpyruvate. Crystals contain a tetramer in the asymmetric unit. A water molecule occupies the presumed binding site for the phosphate group of 4-erythrose 4-phosphate | Aeropyrum pernix |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dipicolinic acid | 1 mM, 1 h, 21% residual activity | Aeropyrum pernix | |
EDTA | 10 mM, 1 h, 21% residual activity | Aeropyrum pernix | |
additional information | none of the downstream products of the shikimate biosynthetic pathway tested inhibits the activity of the enzyme | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
D-erythrose 4-phosphate | pH 7.0, 60°C | Aeropyrum pernix | |
0.891 | - |
phosphoenolpyruvate | pH 7.0, 60°C | Aeropyrum pernix |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | enzyme contains 0.8 molar equivalents of Zn2+ per subunit | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29159 | - |
2 * 30000, SDS-PAGE, 2 * 29159, calculated | Aeropyrum pernix |
30000 | - |
2 * 30000, SDS-PAGE, 2 * 29159, calculated | Aeropyrum pernix |
55400 | - |
gel filtration | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9YEJ7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Aeropyrum pernix | 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 30000, SDS-PAGE, 2 * 29159, calculated | Aeropyrum pernix |
Synonyms | Comment | Organism |
---|---|---|
AroG | - |
Aeropyrum pernix |
DAH7PS | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
1 h, 50% residual activity | Aeropyrum pernix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
phosphoenolpyruvate | pH 7.0, 60°C | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.7 | - |
at 60°C | Aeropyrum pernix |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | - |
60°C, 40% of maximum activity | Aeropyrum pernix |