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Literature summary for 2.5.1.54 extracted from

  • Gosset, G.; Bonner, C.A.; Jensen, R.A.
    Microbial origin of plant-type 2-keto-3-deoxy-D-arabino-heptulosonate 7-phosphate synthases, exemplified by the chorismate- and tryptophan-regulated enzyme from Xanthomonas campestris (2001), J. Bacteriol., 183, 4061-4070.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.54

Activating Compound

Activating Compound Comment Organism Structure
additional information not hysteretically activated by dithiols, e.g. DTT Xanthomonas campestris

Cloned(Commentary)

Cloned (Comment) Organism
2 clones M1 and M2, expression in Escherichia coli strain BL21(DE3) and strain AB3248, which is defective in all endogenous isozyme forms, functional complementation of the mutant Xanthomonas campestris

Inhibitors

Inhibitors Comment Organism Structure
chorismate main allosteric feedback inhibitor and regulator for enzyme of class AroAII Xanthomonas campestris
EDTA strong, reversible by dialysis Xanthomonas campestris
Trp minor feedback inhibitor Xanthomonas campestris

Metals/Ions

Metals/Ions Comment Organism Structure
additional information
-
 Xanthomonas campestris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Xanthomonas campestris
-
 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris
-
 strain ATCC 33436
-
Xanthomonas campestris
-
 2 classes of enzymes: class AroAI and AroAII
-

Purification (Commentary)

Purification (Comment) Organism
partial, 19fold, recombinant from Escherichia coli Xanthomonas campestris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.8
-
 recombinant clone M1 in Escherichia coli strain BL21(DE3) Xanthomonas campestris
2.4
-
 partially purified native enzyme Xanthomonas campestris
3.11
-
 recombinant clone M2 in Escherichia coli strain BL21(DE3) Xanthomonas campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Xanthomonas campestris 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
More 2 distinct homology classes: AroAI and AroAII, both being found in bacteria and higher plant chloroplasts, AroAII can be devided into 4 subgroups, phylogenetic evolution Xanthomonas campestris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
52
-
-
 Xanthomonas campestris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2 8.5 native enzyme Xanthomonas campestris