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Literature summary for 2.5.1.54 extracted from

  • Doong, R.L.; Gander, J.E.; Ganson, R.J.; Jensen, R.A.
    The cytosolic isoenzyme of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase in Spinacia oleracea and other higher plants: extreme substrate ambiguity and other properties (1992), Physiol. Plant., 84, 351-360.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.95
-
D-erythrose 4-phosphate cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
2.5
-
D-erythrose cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
3
-
DL-glyceraldehyde 3-phosphate cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
3.3
-
L-glyceraldehyde cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
3.5
-
D-glyceraldehyde cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
3.6
-
glyoxylate cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
5.1
-
L-erythrose cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
8.4
-
D-threose cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
8.6
-
glycolaldehyde cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea
13.9
-
L-threose cytosolic isozyme DS-Co, pH 8.6, 37°C Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast chloroplastidic isoenzyme DS-Mn Spinacia oleracea 9507
-
cytosol
-
Nicotiana sylvestris 5829
-
cytosol cytosolic isoenzyme DS-Co Spinacia oleracea 5829
-
cytosol cytosolic isoenzyme DS-Co Solanum tuberosum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
Solanum tuberosum
Co2+
-
Vigna radiata
Co2+ absolute requirement for divalent metal, 70% of the activation with Mg2+ Spinacia oleracea
Mg2+
-
Solanum tuberosum
Mg2+ absolute requirement for divalent metal, Mg2+ is most effective Spinacia oleracea
Mn2+
-
Solanum tuberosum
Mn2+
-
Vigna radiata
Mn2+ absolute requirement for divalent metal, 15% of the activation with Mg2+ Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
385000 443000 gel filtration Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Nicotiana sylvestris
-
cytosolic isozyme
-
Pisum sativum
-
cytosolic isozyme Ds-Co
-
Solanum tuberosum
-
cytosolic isozyme Ds-Co
-
Spinacia oleracea
-
cytosolic isozyme Ds-Co
-
Vigna radiata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Spinacia oleracea
partial Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Solanum tuberosum
-
cell culture
-
Nicotiana sylvestris
-
cell suspension culture
-
Solanum tuberosum
-
cell suspension culture
-
Nicotiana sylvestris
-
leaf
-
Pisum sativum
-
seedling
-
Spinacia oleracea
-
seedling
-
Solanum tuberosum
-
seedling
-
Vigna radiata
-
tuber
-
Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.8
-
partially purified cytosolic isozyme, + 10 mM Mg2+ Pisum sativum

Storage Stability

Storage Stability Organism
-80°C, isoenzyme DS-Co, stable for at least several months Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D,L-lyxose D-lyxose or L-lyxose, at 1.8% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-D,L-galacto-octonate + phosphate
-
?
phosphoenolpyruvate + D-arabinose at 0.7% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-D-gluco-octonate + phosphate
-
?
phosphoenolpyruvate + D-arabinose 5-phosphate at 8% of the activity with D-erythrose 4-phosphate Spinacia oleracea 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose at 93% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-D-arabino-heptonate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Spinacia oleracea 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
ir
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Pisum sativum 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Solanum tuberosum 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Vigna radiata 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
Nicotiana sylvestris 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
?
phosphoenolpyruvate + D-glucose 6-phosphate at 0.5% of the activity with D-erythrose 4-phosphate Spinacia oleracea ?
-
?
phosphoenolpyruvate + D-glyceraldehyde at 176% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-D-threo-hexonate + phosphate
-
?
phosphoenolpyruvate + D-ribulose 5-phosphate at 12% of the activity with D-erythrose 4-phosphate Spinacia oleracea ?
-
?
phosphoenolpyruvate + D-threose at 92% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-D-xylo-heptonate + phosphate
-
?
phosphoenolpyruvate + D-xylose at 1% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-D-ido-octonate + phosphate
-
?
phosphoenolpyruvate + DL-glyceraldehyde 3-phosphate at 142% of the activity with D-erythrose 4-phosphate Spinacia oleracea pyruvate + phosphate
-
?
phosphoenolpyruvate + glycolaldehyde at 245% of the activity with D-erythrose 4-phosphate Spinacia oleracea (S)-2-oxo-4,5-dihydroxypentanoate + phosphate
-
?
phosphoenolpyruvate + glyoxylate at 205% of the activity with D-erythrose 4-phosphate Spinacia oleracea S-4-hydroxy-2-oxo-1,5-pentanedioate + R-4-hydroxy-2-oxo-1,5-pentanedioate + phosphate
-
?
phosphoenolpyruvate + L-erythrose at 70% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-L-arabino-heptonate + phosphate
-
?
phosphoenolpyruvate + L-glyceraldehyde at 212% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-L-threo-hexonate + phosphate
-
?
phosphoenolpyruvate + L-threose at 52% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-L-xylo-heptonate + phosphate
-
?
phosphoenolpyruvate + L-xylose at 1% of the activity with D-erythrose 4-phosphate Spinacia oleracea 2-dehydro-3-deoxy-L-ido-octonate + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Spinacia oleracea
37
-
assay at Pisum sativum
37
-
assay at Solanum tuberosum
37
-
assay at Nicotiana sylvestris
47 49
-
Spinacia oleracea
47 49
-
Solanum tuberosum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
isoenzyme Ds-Co, 95% loss of activity in absence of phosphoenolpyruvate, 1.1 mM phosphoenolpyruvate almost completely protects from inactivation Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 chloroplastic isoenzyme DS-Mn Spinacia oleracea
8.6
-
assay at Spinacia oleracea
8.6
-
assay at Pisum sativum
8.6
-
assay at Solanum tuberosum
8.6
-
assay at Nicotiana sylvestris
8.8
-
-
Vigna radiata
9.2
-
cytosolic isoenzyme DS-Co Spinacia oleracea