KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.95 | - |
D-erythrose 4-phosphate | cytosolic isozyme DS-Co, pH 8.6, 37°C | Spinacia oleracea | |
2.5 | - |
D-erythrose | cytosolic isozyme DS-Co, pH 8.6, 37°C | Spinacia oleracea | |
3 | - |
DL-glyceraldehyde 3-phosphate | cytosolic isozyme DS-Co, pH 8.6, 37°C | Spinacia oleracea | |
3.3 | - |
L-glyceraldehyde | cytosolic isozyme DS-Co, pH 8.6, 37°C | Spinacia oleracea | |
3.5 | - |
D-glyceraldehyde | cytosolic isozyme DS-Co, pH 8.6, 37°C | Spinacia oleracea | |
3.6 | - |
glyoxylate | cytosolic isozyme DS-Co, pH 8.6, 37°C | Spinacia oleracea | |
5.1 | - |
L-erythrose | cytosolic isozyme DS-Co, pH 8.6, 37°C | Spinacia oleracea | |
8.4 | - |
D-threose | cytosolic isozyme DS-Co, pH 8.6, 37°C | Spinacia oleracea | |
8.6 | - |
glycolaldehyde | cytosolic isozyme DS-Co, pH 8.6, 37°C | Spinacia oleracea | |
13.9 | - |
L-threose | cytosolic isozyme DS-Co, pH 8.6, 37°C | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | chloroplastidic isoenzyme DS-Mn | Spinacia oleracea | 9507 | - |
cytosol | - |
Nicotiana sylvestris | 5829 | - |
cytosol | cytosolic isoenzyme DS-Co | Spinacia oleracea | 5829 | - |
cytosol | cytosolic isoenzyme DS-Co | Solanum tuberosum | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Solanum tuberosum | |
Co2+ | - |
Vigna radiata | |
Co2+ | absolute requirement for divalent metal, 70% of the activation with Mg2+ | Spinacia oleracea | |
Mg2+ | - |
Solanum tuberosum | |
Mg2+ | absolute requirement for divalent metal, Mg2+ is most effective | Spinacia oleracea | |
Mn2+ | - |
Solanum tuberosum | |
Mn2+ | - |
Vigna radiata | |
Mn2+ | absolute requirement for divalent metal, 15% of the activation with Mg2+ | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
385000 | 443000 | gel filtration | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana sylvestris | - |
cytosolic isozyme | - |
Pisum sativum | - |
cytosolic isozyme Ds-Co | - |
Solanum tuberosum | - |
cytosolic isozyme Ds-Co | - |
Spinacia oleracea | - |
cytosolic isozyme Ds-Co | - |
Vigna radiata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Spinacia oleracea |
partial | Solanum tuberosum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Solanum tuberosum | - |
cell culture | - |
Nicotiana sylvestris | - |
cell suspension culture | - |
Solanum tuberosum | - |
cell suspension culture | - |
Nicotiana sylvestris | - |
leaf | - |
Pisum sativum | - |
seedling | - |
Spinacia oleracea | - |
seedling | - |
Solanum tuberosum | - |
seedling | - |
Vigna radiata | - |
tuber | - |
Solanum tuberosum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.8 | - |
partially purified cytosolic isozyme, + 10 mM Mg2+ | Pisum sativum |
Storage Stability | Organism |
---|---|
-80°C, isoenzyme DS-Co, stable for at least several months | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D,L-lyxose | D-lyxose or L-lyxose, at 1.8% of the activity with D-erythrose 4-phosphate | Spinacia oleracea | 2-dehydro-3-deoxy-D,L-galacto-octonate + phosphate | - |
? | |
phosphoenolpyruvate + D-arabinose | at 0.7% of the activity with D-erythrose 4-phosphate | Spinacia oleracea | 2-dehydro-3-deoxy-D-gluco-octonate + phosphate | - |
? | |
phosphoenolpyruvate + D-arabinose 5-phosphate | at 8% of the activity with D-erythrose 4-phosphate | Spinacia oleracea | 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose | at 93% of the activity with D-erythrose 4-phosphate | Spinacia oleracea | 2-dehydro-3-deoxy-D-arabino-heptonate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Spinacia oleracea | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
ir | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Pisum sativum | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Solanum tuberosum | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Vigna radiata | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Nicotiana sylvestris | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-glucose 6-phosphate | at 0.5% of the activity with D-erythrose 4-phosphate | Spinacia oleracea | ? | - |
? | |
phosphoenolpyruvate + D-glyceraldehyde | at 176% of the activity with D-erythrose 4-phosphate | Spinacia oleracea | 2-dehydro-3-deoxy-D-threo-hexonate + phosphate | - |
? | |
phosphoenolpyruvate + D-ribulose 5-phosphate | at 12% of the activity with D-erythrose 4-phosphate | Spinacia oleracea | ? | - |
? | |
phosphoenolpyruvate + D-threose | at 92% of the activity with D-erythrose 4-phosphate | Spinacia oleracea | 2-dehydro-3-deoxy-D-xylo-heptonate + phosphate | - |
? | |
phosphoenolpyruvate + D-xylose | at 1% of the activity with D-erythrose 4-phosphate | Spinacia oleracea | 2-dehydro-3-deoxy-D-ido-octonate + phosphate | - |
? | |
phosphoenolpyruvate + DL-glyceraldehyde 3-phosphate | at 142% of the activity with D-erythrose 4-phosphate | Spinacia oleracea | pyruvate + phosphate | - |
? | |
phosphoenolpyruvate + glycolaldehyde | at 245% of the activity with D-erythrose 4-phosphate | Spinacia oleracea | (S)-2-oxo-4,5-dihydroxypentanoate + phosphate | - |
? | |
phosphoenolpyruvate + glyoxylate | at 205% of the activity with D-erythrose 4-phosphate | Spinacia oleracea | S-4-hydroxy-2-oxo-1,5-pentanedioate + R-4-hydroxy-2-oxo-1,5-pentanedioate + phosphate | - |
? | |
phosphoenolpyruvate + L-erythrose | at 70% of the activity with D-erythrose 4-phosphate | Spinacia oleracea | 2-dehydro-3-deoxy-L-arabino-heptonate + phosphate | - |
? | |
phosphoenolpyruvate + L-glyceraldehyde | at 212% of the activity with D-erythrose 4-phosphate | Spinacia oleracea | 2-dehydro-3-deoxy-L-threo-hexonate + phosphate | - |
? | |
phosphoenolpyruvate + L-threose | at 52% of the activity with D-erythrose 4-phosphate | Spinacia oleracea | 2-dehydro-3-deoxy-L-xylo-heptonate + phosphate | - |
? | |
phosphoenolpyruvate + L-xylose | at 1% of the activity with D-erythrose 4-phosphate | Spinacia oleracea | 2-dehydro-3-deoxy-L-ido-octonate + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Spinacia oleracea |
37 | - |
assay at | Pisum sativum |
37 | - |
assay at | Solanum tuberosum |
37 | - |
assay at | Nicotiana sylvestris |
47 | 49 | - |
Spinacia oleracea |
47 | 49 | - |
Solanum tuberosum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
isoenzyme Ds-Co, 95% loss of activity in absence of phosphoenolpyruvate, 1.1 mM phosphoenolpyruvate almost completely protects from inactivation | Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | chloroplastic isoenzyme DS-Mn | Spinacia oleracea |
8.6 | - |
assay at | Spinacia oleracea |
8.6 | - |
assay at | Pisum sativum |
8.6 | - |
assay at | Solanum tuberosum |
8.6 | - |
assay at | Nicotiana sylvestris |
8.8 | - |
- |
Vigna radiata |
9.2 | - |
cytosolic isoenzyme DS-Co | Spinacia oleracea |