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Literature summary for 2.5.1.51 extracted from

  • Murakoshi, I.; Ikegami, F.; Hinuma, Y.; Hanma, Y.
    Purification and characterization of beta-(pyrazol-1-yl)-L-alanine synthase from Citrullus vulgaris (1984), Phytochemistry, 23, 973-977.
No PubMed abstract available
Search for more literature for 2.5.1.51

General Stability

General Stability Organism
freeze-thawing or lyophilization inactivates Citrullus lanatus subsp. vulgaris
sensitive to freezing even in the presence of 20% glycerol Citrullus lanatus subsp. vulgaris

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine 50 mM, almost complete inhibition Citrullus lanatus subsp. vulgaris
KCN 10 mM, almost complete inhibition Citrullus lanatus subsp. vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5
-
 O-acetylserine
-
 Citrullus lanatus subsp. vulgaris
74
-
 pyrazole
-
 Citrullus lanatus subsp. vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000, SDS-PAGE Citrullus lanatus subsp. vulgaris
58000
-
 gel filtration Citrullus lanatus subsp. vulgaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O3-acetyl-L-serine + pyrazole Citrullus lanatus subsp. vulgaris involved in biosynthesis of heterocyclic beta-substituted alanines 3-(pyrazol-1-yl)-L-alanine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Citrullus lanatus subsp. vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Citrullus lanatus subsp. vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
additional information barely detectable in dry seeds Citrullus lanatus subsp. vulgaris
-
seedling cotyledons removed, 1 week old Citrullus lanatus subsp. vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.355
-
-
 Citrullus lanatus subsp. vulgaris

Storage Stability

Storage Stability Organism
0-2°C, stable for at least 1 month Citrullus lanatus subsp. vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-succinyl-L-serine + pyrazole at 16% the rate of O-acetyl-L-serine Citrullus lanatus subsp. vulgaris 3-(pyrazol-1-yl)-L-alanine + succinate
-
 ?
O-sulfo-L-serine + pyrazole at 2.5% the rate of O-acetyl-L-serine Citrullus lanatus subsp. vulgaris 3-(pyrazol-1-yl)-L-alanine + SO42-
-
 ?
O3-acetyl-L-serine + 3-amino-1,2,4-triazole at 11% the rate of pyrazole Citrullus lanatus subsp. vulgaris 3-(3-amino-1,2,4-triazol-1-yl)-L-alanine + acetate
-
 ?
O3-acetyl-L-serine + N-hydroxyurea at 9% the rate of pyrazole Citrullus lanatus subsp. vulgaris O-ureidoserine + acetate
-
 ?
O3-acetyl-L-serine + pyrazole highly specific Citrullus lanatus subsp. vulgaris 3-(pyrazol-1-yl)-L-alanine + acetate
-
 ?
O3-acetyl-L-serine + pyrazole involved in biosynthesis of heterocyclic beta-substituted alanines Citrullus lanatus subsp. vulgaris 3-(pyrazol-1-yl)-L-alanine + acetate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE Citrullus lanatus subsp. vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 7.4
-
 Citrullus lanatus subsp. vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 about half-maximal activity at pH 6.5 and 8.5 Citrullus lanatus subsp. vulgaris

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate requirement, enzyme bound, 1 mol pyridoxal 5'-phosphate per mol subunit, further increase of activity by 40% by adding pyridoxal phosphate Citrullus lanatus subsp. vulgaris