Literature summary for 2.5.1.49 extracted from

Kulikova, V.V.; Revtovich, S.V.; Bazhulina, N.P.; Anufrieva, N.V.; Kotlov, M.I.; Koval, V.S.; Morozova, E.A.; Hayashi, H.; Belyi, Y.F.; Demidkina, T.V.
Identification of O-acetylhomoserine sulfhydrylase, a putative enzyme responsible for methionine biosynthesis in Clostridioides difficile Gene cloning and biochemical characterizations (2019), IUBMB Life, 71, 1815-1823 .

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Inhibitors

Inhibitors	Comment	Organism	Structure
L-methionine	product inhibition	Clostridioides difficile
L-propargylglycine	irreversible, rapid inactivation; suicide inhibitor	Clostridioides difficile
Ngamma-acetyl-L-2,4-diaminobutyric acid	competitive; competitive and reversible inhibition	Clostridioides difficile

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.4	-	hydrogen sulfide	pH 7.5, 25°C	Clostridioides difficile
0.4	-	methanethiol	pH 7.5, 25°C	Clostridioides difficile
0.6	-	O-acetyl-L-homoserine	pH 7.5, 25°C	Clostridioides difficile

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
180000	-	gel filtration	Clostridioides difficile
180000	-	gel fitration	Clostridioides difficile

Organism

Organism	UniProt	Comment	Textmining
Clostridioides difficile	Q187D4	-	-
Clostridioides difficile 630	Q187D4	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
50	-	pH 7.5, 25°C	Clostridioides difficile

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
O-acetyl-L-homoserine + hydrogen sulfide	-	Clostridioides difficile	L-homocysteine + acetate	-	?
O-acetyl-L-homoserine + hydrogen sulfide	-	Clostridioides difficile 630	L-homocysteine + acetate	-	?
O-acetyl-L-homoserine + methanethiol	-	Clostridioides difficile	L-methionine + acetate	-	?
O-acetyl-L-homoserine + methanethiol	-	Clostridioides difficile 630	L-methionine + acetate	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 46000, SDS-PAGE, 4 * 46355, calculated from sequence	Clostridioides difficile

Synonyms

Synonyms	Comment	Organism
MetY	-	Clostridioides difficile
OAHS	-	Clostridioides difficile
Q187D4	-	Clostridioides difficile

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
94.12	-	O-acetyl-L-homoserine	pH 7.5, 25°C	Clostridioides difficile
96.65	-	hydrogen sulfide	pH 7.5, 25°C	Clostridioides difficile
96.65	-	methanethiol	pH 7.5, 25°C	Clostridioides difficile

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	higher activity is shown in potassium phosphate buffer compared to sodium phosphate and Tris-HCl buffers	Clostridioides difficile

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Clostridioides difficile
pyridoxal 5'-phosphate	the absorption spectrum of the holoenzyme at pH 7.5 shows a major band in the region of 415-416 nm corresponding to ketoenamine tautomer of the internal aldimines	Clostridioides difficile

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.04	-	Ngamma-acetyl-L-2,4-diaminobutyric acid	pH 7.5, 25°C	Clostridioides difficile
9	-	L-methionine	pH 7.5, 25°C	Clostridioides difficile

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
160	-	O-acetyl-L-homoserine	pH 7.5, 25°C	Clostridioides difficile
240	-	hydrogen sulfide	pH 7.5, 25°C	Clostridioides difficile
240	-	methanethiol	pH 7.5, 25°C	Clostridioides difficile

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Release 2023.1 (January 2023)