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Literature summary for 2.5.1.49 extracted from
- Purification, characterization and gene cloning of thermostable o-acetyl-L-homoserine sulfhydrylase forming gamma-cyano-alpha-aminobutyric acid (2003), J. BIOSCI. BIOENG., 96, 53-58.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Geobacillus stearothermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.87 | - |
O-acetyl-L-homoserine | at 45°C, pH 7.5 | Geobacillus stearothermophilus |  |
| 4.17 | - |
L-homocysteine | at 45°C, pH 7.5 | Geobacillus stearothermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CN- | - |
Geobacillus stearothermophilus | |
| additional information | cyanide ions can be replaced as cosubstrates by hydrogen sulfide, methylmercaptan, ethylmercaptan and phenylmercaptan | Geobacillus stearothermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
SDS-PAGE | Geobacillus stearothermophilus |
| 46850 | - |
calculated from amino acid sequence | Geobacillus stearothermophilus |
| 180000 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| o-acetyl-L-homoserine + CN- | Geobacillus stearothermophilus | glutamine and glutamic acid synthesis pathway | gamma-cyano-alpha-aminobutyric acid + acetate | - |
? | |
| o-acetyl-L-homoserine + CN- | Geobacillus stearothermophilus CN3 | glutamine and glutamic acid synthesis pathway | gamma-cyano-alpha-aminobutyric acid + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
isolated from a hot spring in Japan | - |
| Geobacillus stearothermophilus CN3 | - |
isolated from a hot spring in Japan | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0272 | - |
cell-free extract | Geobacillus stearothermophilus |
| 0.0479 | - |
after heat treatment | Geobacillus stearothermophilus |
| 0.37 | - |
after DEAE-cellufine A-500m treatment | Geobacillus stearothermophilus |
| 14.5 | - |
after Phenyl-Toyopearl 650M treatment | Geobacillus stearothermophilus |
| 17.2 | - |
after sephacryl S-200 HR treatment | Geobacillus stearothermophilus |
| 29.9 | - |
recombinant enzyme purified from Escherichia coli | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + KCN | inactive | Geobacillus stearothermophilus | ? | - |
? | |
| L-cysteine + KCN | inactive | Geobacillus stearothermophilus CN3 | ? | - |
? | |
| L-glutamic acid + KCN | inactive | Geobacillus stearothermophilus | ? | - |
? | |
| L-glutamic acid + KCN | inactive | Geobacillus stearothermophilus CN3 | ? | - |
? | |
| L-glutamine + KCN | inactive | Geobacillus stearothermophilus | ? | - |
? | |
| L-glutamine + KCN | inactive | Geobacillus stearothermophilus CN3 | ? | - |
? | |
| L-homocysteine + KCN | - |
Geobacillus stearothermophilus | gamma-cyano-alpha-aminobutyric acid + H2S | - |
? | |
| L-homocysteine + KCN | - |
Geobacillus stearothermophilus CN3 | gamma-cyano-alpha-aminobutyric acid + H2S | - |
? | |
| L-homoserine + KCN | inactive | Geobacillus stearothermophilus | ? | - |
? | |
| L-serine + KCN | inactive | Geobacillus stearothermophilus | ? | - |
? | |
| o-acetyl-L-homoserine + CN- | glutamine and glutamic acid synthesis pathway | Geobacillus stearothermophilus | gamma-cyano-alpha-aminobutyric acid + acetate | - |
? | |
| o-acetyl-L-homoserine + CN- | glutamine and glutamic acid synthesis pathway | Geobacillus stearothermophilus CN3 | gamma-cyano-alpha-aminobutyric acid + acetate | - |
? | |
| o-acetyl-L-homoserine + KCN | - |
Geobacillus stearothermophilus | gamma-cyano-alpha-aminobutyric acid + acetate | - |
? | |
| o-acetyl-L-serine + KCN | inactive | Geobacillus stearothermophilus | ? | - |
? | |
| o-phospho-L-serine + KCN | inactive | Geobacillus stearothermophilus | ? | - |
? | |
| o-succinyl-L-homoserine + KCN | inactive | Geobacillus stearothermophilus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Geobacillus stearothermophilus |
| tetramer | 4 * 43000, gel filtration, SDS-PAGE | Geobacillus stearothermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gamma-cyano-alpha-aminobutyric acid synthase | - |
Geobacillus stearothermophilus |
| o-acetyl-L-homoserine sulfhydrylase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
no loss of activity after incubation at temperatures up to 60°C for 30 min at pH 7.5 | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
using Tris-HCl buffer | Geobacillus stearothermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10.5 | stable on incubation at 60°C for 30 min | Geobacillus stearothermophilus |
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