Cloned (Comment) | Organism |
---|---|
MET17/MET25 gene cloned and overexpressed in Escherichia coli | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
sensitive to trypsin digestion | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hydroxylamine hydrochloride | - |
Saccharomyces cerevisiae | |
L-Penicillamine | - |
Saccharomyces cerevisiae | |
phenylhydrazine | - |
Saccharomyces cerevisiae | |
Semicarbazide | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.7 | - |
O-acetyl-L-homoserine | wild-type enzyme | Saccharomyces cerevisiae | |
9 | - |
O-acetyl-L-homoserine | recombinant enzyme | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and recombinant enzyme | Saccharomyces cerevisiae |