Crystallization (Comment) | Organism |
---|---|
to 2.2 A resolution | Bacillus anthracis |
Protein Variants | Comment | Organism |
---|---|---|
A72S | mutant produces more H2S than wild-type | Bacillus anthracis |
E220R | not able to release H2S | Bacillus anthracis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.168 | - |
O-acetyl-L-serine | wild-type, pH 8.2, 37°C | Bacillus anthracis | |
1.65 | - |
O-acetyl-L-serine | mutant A72S, pH 8.2, 37°C | Bacillus anthracis | |
2.05 | - |
O-acetyl-L-serine | mutant E220R, pH 8.2, 37°C | Bacillus anthracis | |
6.18 | - |
O-acetyl-L-serine | production of H2S, wild-type, pH 8.2, 37°C | Bacillus anthracis | |
9.01 | - |
O-acetyl-L-serine | production of H2S, mutant A72S, pH 8.2, 37°C | Bacillus anthracis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | A0A1J9VES8 | enzyme shows activities of both EC 4.2.1.22 and 2.5.1.47 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + H2O | - |
Bacillus anthracis | L-serine + H2S | - |
? | |
L-cysteine + L-homocysteine | - |
Bacillus anthracis | L-cystathionine + H2S | - |
? | |
additional information | no substrate: L-serine | Bacillus anthracis | ? | - |
? | |
O-acetyl-L-serine | - |
Bacillus anthracis | L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + H2S | - |
Bacillus anthracis | L-cysteine + acetic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31400, SDS-PAGE | Bacillus anthracis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
O-acetyl-L-serine | production of H2S, wild-type, pH 8.2, 37°C | Bacillus anthracis | |
0.9 | - |
O-acetyl-L-serine | production of H2S, mutant A72S, pH 8.2, 37°C | Bacillus anthracis | |
4.46 | - |
O-acetyl-L-serine | wild-type, pH 8.2, 37°C | Bacillus anthracis | |
4.88 | - |
O-acetyl-L-serine | mutant A72S, pH 8.2, 37°C | Bacillus anthracis | |
5.46 | - |
O-acetyl-L-serine | mutant E220R, pH 8.2, 37°C | Bacillus anthracis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | cofactor is highly flexible due to the active site being wide open | Bacillus anthracis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
O-acetyl-L-serine | production of H2S, mutant A72S, pH 8.2, 37°C | Bacillus anthracis | |
0.11 | - |
O-acetyl-L-serine | production of H2S, wild-type, pH 8.2, 37°C | Bacillus anthracis | |
2.67 | - |
O-acetyl-L-serine | mutant E220R, pH 8.2, 37°C | Bacillus anthracis | |
2.96 | - |
O-acetyl-L-serine | mutant A72S, pH 8.2, 37°C | Bacillus anthracis | |
3.82 | - |
O-acetyl-L-serine | wild-type, pH 8.2, 37°C | Bacillus anthracis | |
10.56 | - |
L-homocysteine | wild-type, production of H2S, pH 8.2, 37°C | Bacillus anthracis |