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Literature summary for 2.5.1.47 extracted from

  • Birke, H.; Heeg, C.; Wirtz, M.; Hell, R.
    Successful fertilization requires the presence of at least one major O-acetylserine(thiol)lyase for cysteine synthesis in pollen of Arabidopsis (2013), Plant Physiol., 163, 959-972.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene OASA1 Arabidopsis thaliana
gene OASB1 Arabidopsis thaliana
gene OASC1 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information generation of a OAS-TL A mutant by T-DNA insertion. Generation major isozyme OAS-TL double loss-of-function mutants oastlBC A+/+, oastlAC B+/+, and oastlAB C+/+, leaving only one major OAS-TL in the cytosol, in the plastids, and in the mitochondria, respectively. Extractable OAS-TL activity is not altered in the oastlBC A+/+ mutant compared with the wild-type, while OAS-TL activities are decreased to 30% and 3% in the oastlAC B+/+ and oastlAB C+/+ mutants, respectively. Phenotypes, overview. The incorporation of sulfur into thiols but not thiol steady-state levels is decreased in mutants lacking cytosolic OAS-TL A Arabidopsis thaliana
additional information generation of a OAS-TL B mutant by T-DNA insertion. Generation major isozyme OAS-TL double loss-of-function mutants oastlBC A+/+, oastlAC B+/+, and oastlAB C+/+, leaving only one major OAS-TL in the cytosol, in the plastids, and in the mitochondria, respectively. Extractable OAS-TL activity is not altered in the oastlBC A+/+ mutant compared with the wild-type, while OAS-TL activities are decreased to 30% and 3% in the oastlAC B+/+ and oastlAB C+/+ mutants, respectively. Phenotypes, overview Arabidopsis thaliana
additional information generation of a OAS-TL C mutant by T-DNA insertion. Generation major isozyme OAS-TL double loss-of-function mutants oastlBC A+/+, oastlAC B+/+, and oastlAB C+/+, leaving only one major OAS-TL in the cytosol, in the plastids, and in the mitochondria, respectively. Extractable OAS-TL activity is not altered in the oastlBC A+/+ mutant compared with the wild-type, while OAS-TL activities are decreased to 30% and 3% in the oastlAC B+/+ and oastlAB C+/+ mutants, respectively. Phenotypes, overview. Lack of mitochondrial OAS-TL C diminishes the incorporation of OAS into thiols without affecting thiol steady-state levels Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast isozyme OAS-TL B Arabidopsis thaliana 9507
-
cytosol isozyme OAS-TL A Arabidopsis thaliana 5829
-
mitochondrion isozyme OAS-TL C Arabidopsis thaliana 5739
-
additional information the major isoforms, OAS-TL A, OAS-TL B, and OAS-TL C, catalyze the formation of Cys by combining O-acetylserine and sulfide in the cytosol, the plastids, and the mitochondria, respectively. Subcellular localization of OAS-TL proteins is more important for efficient Cys synthesis than total cellular OAS-TL activity in leaves Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-acetyl-L-serine + hydrogen sulfide Arabidopsis thaliana
-
L-cysteine + acetate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P47998 gene OASA1
-
Arabidopsis thaliana P47999 gene OASB1
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Arabidopsis thaliana Q43725 gene OASC1
-

Source Tissue

Source Tissue Comment Organism Textmining
gametophyte male and female Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
pollen
-
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-serine + hydrogen sulfide
-
Arabidopsis thaliana L-cysteine + acetate
-
?

Synonyms

Synonyms Comment Organism
(OAS-TL)-like protein
-
Arabidopsis thaliana
cysteine synthase 1 UniProt Arabidopsis thaliana
cysteine synthase, chloroplastic/chromoplastic UniProt Arabidopsis thaliana
cysteine synthase, mitochondrial UniProt Arabidopsis thaliana
O-acetylserine(thiol)lyase
-
Arabidopsis thaliana
OAS-TL A
-
Arabidopsis thaliana
OAS-TL B
-
Arabidopsis thaliana
OAS-TL C
-
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction Cys can be supplied by the mother plant for the development of female gametophytes lacking OAS-TL activity. In contrast, the presence of at least one functional OAS-TL isoform is essential in the male gametophyte. Only the absence of both isozymes OAS-TL A and OAS-TL C results in a decreased incorporation of sulfur and the carbon/nitrogen backbone into thiols, which also causes lower thiol steady-state levels. A segregation pattern can only be explained by a gamete-lethal phenotype of the oastlABC triple mutant. Phenotypes of mutants in the generative phase of the life cycle, overview Arabidopsis thaliana
metabolism the subcellular compartmentation of Cys precursor formation is a remarkable feature of Cys synthesis in higher plants that implies a high degree of regulation between the participating compartments. Contribution of additional OAS-TL-like proteins to Cys synthesis, overview Arabidopsis thaliana
physiological function the major isoforms, OAS-TL A, OAS-TL B, and OAS-TL C, catalyze the formation of Cys by combining O-acetylserine and sulfide in the cytosol, the plastids, and the mitochondria, respectively. Subcellular localization of OAS-TL proteins is more important for efficient Cys synthesis than total cellular OAS-TL activity in leaves Arabidopsis thaliana