Cloned (Comment) | Organism |
---|---|
gene Cy-OAS-TL, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) pLYsS | Leucaena leucocephala |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics and modeling | Leucaena leucocephala | |
1.46 | - |
hydrogen sulfide | pH 7.5, 22°C, recombinant enzyme, Lineweaver-Burke model | Leucaena leucocephala | |
1.85 | - |
hydrogen sulfide | pH 7.5, 22°C, recombinant enzymem, Michaelis-Menten model | Leucaena leucocephala |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Leucaena leucocephala | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
- |
Leucaena leucocephala |
40000 | - |
- |
Leucaena leucocephala |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + hydrogen sulfide | Leucaena leucocephala | - |
L-cysteine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucaena leucocephala | W5S3I4 | gene Cy-OAS-TL | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) pLYsS by nickel affinity chhromatography | Leucaena leucocephala |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no synthesis of mimosine. The enzyme is specific for cysteine synthesis. The recombinant enzyme is active with or without the leader peptide | Leucaena leucocephala | ? | - |
? | |
O-acetyl-L-serine + hydrogen sulfide | - |
Leucaena leucocephala | L-cysteine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, recombinant His-tagged enzyme, SDS-PAGE, x * 34000, about, sequence calculation of untagged enzyme | Leucaena leucocephala |
Synonyms | Comment | Organism |
---|---|---|
O-acetylserine (thiol) lyase | - |
Leucaena leucocephala |
OAS-TL | - |
Leucaena leucocephala |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Leucaena leucocephala |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Leucaena leucocephala |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Leucaena leucocephala |
General Information | Comment | Organism |
---|---|---|
physiological function | tree legume Leucaena contains a toxic, nonprotein amino acid, mimosine, which is not formed by the enzyme O-acetylserine (thiol) lyase, OAS-TL | Leucaena leucocephala |