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Literature summary for 2.5.1.47 extracted from

  • Ozaki, S.; Nakahara, A.; Sakaguchi, C.
    Mutagenesis of Gln-142 and Phe-143 of O-acetylserine sulfhydrylase (2009), J. Biochem. Mol. Biol. Biophys., 4, 117-124.
No PubMed abstract available
Search for more literature for 2.5.1.47

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a GST-fusion protein Escherichia coli

Protein Variants

Protein Variants Comment Organism
F143A mutant retains one molecule of pyridoxal 5'-phosphate per subunit, mutant reacts with O-acetylserine but the rate is significantly smaller, kcat/KM (O-acetylserine): 950/Msec Escherichia coli
F143D mutant retains one molecule of pyridoxal 5'-phosphate per subunit, mutant reacts with O-acetylserine but the rate is significantly smaller, kcat/KM (O-acetylserine): 150/Msec Escherichia coli
F143S mutant retains one molecule of pyridoxal 5'-phosphate per subunit, mutant reacts with O-acetylserine but the rate is significantly smaller, kcat/KM (O-acetylserine): 380/Msec Escherichia coli
F143Y mutant retains one molecule of pyridoxal 5'-phosphate per subunit, reaction with O-acetylserine is inhibited Escherichia coli
Q142A ability of pyridoxal 5'-phosphate binding is not altered, mutant does not react with O-acetylserine Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a GSTrap 4 B column and anion-exchange chromatography Escherichia coli

Synonyms

Synonyms Comment Organism
O-acetylserine sulfhydrylase
-
 Escherichia coli
OASS
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.15
-
 O-acetyl-L-serine mutant F143D Escherichia coli
0.38
-
 O-acetyl-L-serine mutant F143S Escherichia coli
0.95
-
 O-acetyl-L-serine mutant F143A Escherichia coli
15
-
 O-acetyl-L-serine wild-type Escherichia coli